Abstract  We investigated relationships between sestonic chlorophyll (Chl), total phosphorus (TP), and total nitrogen (TN) at 23 sites an 13 streams in the Missouri Ozarks. There was a strong curvilinear relationship between mean sestonic Chi and both mean TP (R-2 = 0.78) and mean TN (R-2 = 0.70). Both models were improved when catchment area was included with either TP (R-2 = 0.90) or TN (R-2 = 0.84). Limited to 17 sites without point source nutrient additions, the relationship between sestonic Chi and both TP and TN was linear Including catchment area strengthened linear models with either TP (R-2 = 0.94) or TN (R-2 = 0.84). Land use (percent row crop or percent forest), together with catchment size, was also a good predictor of sestonic Chl in Ozark streams without point sources. When catchment area and TP or TN were used to predict sestonic Chi on specific dates following catastrophic flooding, models were less accurate than those based on long-term averages, but still explained 55-74% of the variance in sestonic Chi. Our results demonstrate that sestonic Chi is closely associated with nutrients and catchment area in Ozark streams and that nutrient - sestonic Chl models may have broad application in running waters.

Abstract  Biotic indices for algae, macroinvertebrates, and fish assemblages can be effective for monitoring stream enrichment, but little is known regarding the value of the three assemblages for detecting perturbance as a consequence of low-level nutrient enrichment. In the summer of 2006, we collected nutrient and biotic samples from 30 wadeable Ozark streams that spanned a nutrient-concentration gradient from reference to moderately enriched conditions. Seventy-three algal metrics, 62 macroinvertebrate metrics, and 60 fish metrics were evaluated for each of the three biotic indices. After a group of candidate metrics had been identified with multivariate analysis, correlation procedures and scatter plots were used to identify the four metrics having strongest relations to a nutrient index calculated from log transformed and normalized total nitrogen and total phosphorus concentrations. The four metrics selected for each of the three biotic indices were: algae-the relative abundance of most tolerant diatoms, the combined relative abundance of three species of Cymbella, mesosaprobic algae percent taxa richness, and the relative abundance of diatoms that are obligate nitrogen heterotrophs; macroinvertebrate-the relative abundance of intolerant organisms, Baetidae relative abundance, moderately tolerant taxa richness, and insect biomass; fish-herbivore and detritivore taxa richness, pool species relative abundance, fish catch per unit effort, and black bass (Micropterus spp.) relative abundance. All three biotic indices were negatively correlated to nutrient concentrations but the algal index had a higher correlation (rho = -0.89) than did the macroinvertebrate and fish indices (rho = -0.63 and -0.58, respectively). Biotic index scores were lowest and nutrient concentrations were highest for streams with basins having the highest poultry and cattle production. Because of the availability of litter for fertilizer and associated increases in grass and hay production, cattle feeding capacity increases with poultry production. Studies are needed that address the synergistic effect of poultry and cattle production on Ozark streams in high production areas before ecological risks can be adequately addressed. Published by Elsevier Ltd.

Abstract  The Red River is a transboundary, multijurisdictional basin where water-quality standards are often different across state lines. The state agencies with USEPA Region VI focused resources to organize water-quality data from within this basin and have it statistically analyzed to evaluate the relationships between nutrients and sestonic chlorophyll-a (chl-a). There were 152 sites within the Red River basin that had nutrient and sestonic chl-a measurements; these sites were narrowed down to 132 when a minimum number of observations was required. Sestonic chl-a levels increased with increasing nutrient concentrations; these regressions were used to predict nutrient concentrations at 10 µg chl-a L-1. Total nitrogen (TN) and phosphorus (TP) concentrations (at 10 µg chl-a L-1) varied across the Red River basin and its ecoregions from 0.10 to 0.22 mg TP L-1 and 0.75 to 2.11 mg TN L-1. Nutrient thresholds were also observed with sestonic chl-a at 0.14 mg TP L-1 and 0.74 mg TN L-1 using categorical and regression tree analysis (CART). The CART analysis also revealed that hierarchical structure was important when attempting to predict sestonic chl-a from TN, TP, and conductivity. The ranges of TN and TP concentrations that resulted in chl-a concentrations that exceeded 10 µg chl-a L-1 were similar in magnitude to the threshold in TN and TP that resulted in increased sestonic chl-a. This corroborating evidence provides useful guidance to the states with jurisdiction within the Red River basin for establishing nutrient criteria, which may be similar when the Red River and its tributaries cross political boundaries.

Abstract  The influence of environmental factors on biotic responses to nutrients was examined in three diverse agricultural regions of the United States. Seventy wadeable sites were selected along an agricultural land use gradient while minimizing natural variation within each region. Nutrients, habitat, algae, macroinvertebrates, and macrophyte cover were sampled during a single summer low-flow period in 2006 or 2007. Continuous stream stage and water temperature were collected at each site for 30 days prior to sampling. Wide ranges of concentrations were found for total nitrogen (TN) (0.07-9.61 mg/l) and total phosphorus (TP) (< 0.004-0.361 mg/l), but biotic responses including periphytic and sestonic chlorophyll a (RCHL and SCHL, respectively), and percent of stream bed with aquatic macrophyte (AQM) growth were not strongly related to concentrations of TN or TP. Pearson's coefficient of determination (R2) for nutrients and biotic measures across all sites ranged from 0.08 to 0.32 and generally were not higher within each region. The biotic measures (RCHL, SCHL, and AQM) were combined in an index to evaluate eutrophic status across sites that could have different biotic responses to nutrient enrichment. Stepwise multiple regression identified TN, percent canopy, median riffle depth, and daily percent change in stage as significant factors for the eutrophic index (R2 = 0.50, p < 0.001). A TN threshold of 0.48 mg/l was identified where eutrophic index scores became less responsive to increasing TN concentrations, for all sites. Multiple plant growth indicators should be used when evaluating eutrophication, especially when streams contain an abundance of macrophytes.

Abstract  Nutrient concentrations, benthic algal biomass, dissolved oxygen (DO), and pH were measured in 70 or more streams during spring and summer in the Illinois River Watershed (IRW), which crosses the Oklahoma and Arkansas (USA) border, to determine whether injury to streams occurred and if that injury was related to spreading poultry waste on fields. Definitions of injury were based on Oklahoma water quality regulations and scientific literature. Phosphorus and nitrogen concentrations were each independently related to poultry house density (PHD) in watersheds and percent urban land use in watersheds. In addition, phosphorus and nitrogen concentrations were unusually high compared to regions with similar geology and hydrology. Molar N:P ratios were high and indicated that phosphorus was the most likely limiting nutrient. Phosphorus concentrations, as well as PHD and urban land use, were related to algal biomass during spring, but were less related during summer. A threshold response in cover of stream bottoms by nuisance filamentous green algae (NFGA: Cladophora, Rhizoclonium, and Oedogonium) during spring was observed at 27 mu g TP l(-1) using regression tree analysis. Great increases in average NFGA cover (from 4 to 36% cover) occurred with relatively small increases in TP concentration at the 27 mu g TP l(-1) threshold. Average concentrations of DO, variability in DO, and pH during spring were positively related to TP, chlorophyll a, and NFGA cover. Minimum DO during spring and early morning DO during summer were negatively related to TP concentration. Spring pH and summer DO frequently violated water quality requirements for protecting biodiversity that were established by the state of Oklahoma. We conclude that poultry house operations as well as urban activities, independently and interactively, pollute IRW streams with phosphorus, which resulted in injury to aesthetic condition and the potential for injury of biodiversity.

Abstract  During August through September of 1993- 95, 83 periphyton samples were collected at 51 stream sites in the Ozark Plateaus. These sites were categorized into six land-use categories (20 forest, 18 agriculture, 10 mining, 1 urban, 1 urban/ mining, and 1 mix), based on land-use percentages in the basin upstream from the site. Results indicate that periphyton communities of riffles of Ozark streams are affected by natural and land-use related factors. These factors include nutrients, dissolved organic carbon, alkalinity, canopy shading, suspended sediment, embeddedness, stream morphometry, and velocity. For several measures of periphyton communities, statistically significant (p<0.05) differences were found among sites assigned to agriculture, forest, and mining categories. Blue-green algae biovolume, relative abundance of blue-green algae, relative biovolume of diatoms, relative abundance of oligotrophic algae, relative abundance of tolerant taxa, and condition index values were among the measures that differed among land-use categories. Although no environmental factors were significantly correlated with total biovolume, several factors were significantly correlated with biovolume of blue-green algae or biovolume of diatoms. Biovolume of blue-green algae was correlated with percent agriculture land use. Biovolume of diatoms was correlated with orthophosphate, total phosphorus, alkalinity, velocity, embeddedness, and dissolved organic carbon. Diatoms often composed the largest percentage of the biovolume (relative biovolume). Diatom relative biovolume was much higher at mining sites (generally 75 to 90 percent of the total biovolume) than at forest or agriculture sites (generally 15 to 80 percent) and was correlated with several factors, including many land-use related factors. The diatoms Cymbella affinis and Cymbella delicatula and the blue-green algae Calothrix often were the most common (relative abundance and relative biovolume) algae in samples. Detrended correspondence analysis (DCA) and hierarchical cluster analyses results indicated differences among land-use category sites. The DCA results were correlated with a number of land-use related factors and channel morphometry. Grazers (specifically, snails and stonerollers) are related to periphyton biovolume and community composition. Total periphyton and diatom biovolume typically were highest at sites where snail density was lowest. Lower relative abundances of diatoms usually occurred at sites with higher snail densities and stoneroller relative abundances.

Abstract  Research was conducted at 28-30 sites within eight study areas across the United States along a gradient of nutrient enrichment/agricultural land use between 2003 and 2007. Objectives were to test the application of an agricultural intensity index (AG-Index) and compare among various invertebrate and algal metrics to determine indicators of nutrient enrichment nationally and within three regions. The agricultural index was based on total nitrogen and phosphorus input to the watershed, percent watershed agriculture, and percent riparian agriculture. Among data sources, agriculture within riparian zone showed significant differences among values generated from remote sensing or from higher resolution orthophotography; median values dropped significantly when estimated by orthophotography. Percent agriculture in the watershed consistently had lower correlations to invertebrate and algal metrics than the developed AG-Index across all regions. Percent agriculture showed fewer pairwise comparisons that were significant than the same comparisons using the AG-Index. Highest correlations to the AG-Index regionally were 0.75 for Ephemeroptera, Plecoptera, and Trichoptera richness (EPTR) and 0.70 for algae Observed/Expected (O/E), nationally the highest was 0.43 for EPTR vs. total nitrogen and 0.62 for algae O/E vs. AG-Index. Results suggest that analysis of metrics at national scale can often detect large differences in disturbance, but more detail and specificity is obtained by analyzing data at regional scales.

Abstract  1. Algal-community metrics were calculated for periphyton samples collected from 976 streams and rivers by the U.S. Geological Survey's National Water-Quality Assessment (NAWQA) Programme during 1993-2001 to evaluate national and regional relations with water chemistry and to compare whether algal-metric values differ significantly among undeveloped and developed land-use classifications.

2. Algal metrics with significant positive correlations with nutrient concentrations included indicators of trophic condition, organic enrichment, salinity, motility and taxa richness. The relative abundance of nitrogen-fixing algae was negatively correlated with nitrogen concentrations, and the abundance of diatom species associated with high dissolved oxygen concentrations was negatively correlated with both nitrogen and phosphorus concentrations. Median algal-metric values and nutrient concentrations were significantly lower at undeveloped sites than those draining agricultural or urban catchments.

3. Total algal biovolume did not differ significantly among major river catchments or land-use classifications, and was only weakly correlated with nitrate (positive) and suspended-sediment (negative) concentrations. Estimates of periphyton chlorophyll a indicated an oligotrophic-mesotrophic boundary of about 21 mg m(-2) and a mesotrophic-eutrophic boundary of about 55 mg m(-2) based on upper and lower quartiles of the biovolume data distribution.

4. Although algal species tolerance to nutrient and organic enrichment is well documented, additional taxonomic and autecological research on sensitive, endemic algal species would further enhance water-quality assessments.

Abstract  As part of the USGS study of nutrient enrichment of streams in agricultural regions throughout the United States, about 30 sites within each of eight study areas were selected to capture a gradient of nutrient conditions. The objective was to develop watershed disturbance predictive models for macroinvertebrate and algal metrics at national and three regional landscape scales to obtain a better understanding of important explanatory variables. Explanatory variables in models were generated from landscape data, habitat, and chemistry. Instream nutrient concentration and variables assessing the amount of disturbance to the riparian zone (e.g., percent row crops or percent agriculture) were selected as most important explanatory variable in almost all boosted regression tree models regardless of landscape scale or assemblage. Frequently, TN and TP concentration and riparian agricultural land use variables showed a threshold type response at relatively low values to biotic metrics modeled. Some measure of habitat condition was also commonly selected in the final invertebrate models, though the variable(s) varied across regions. Results suggest national models tended to account for more general landscape/climate differences, while regional models incorporated both broad landscape scale and more specific local-scale variables.