Dendritic chain reaction: responsive release of hydrogen peroxide upon generation and enzymatic oxidation of methanol
Avital-Shmilovici, M; Shabat, D
| HERO ID | 1037021 |
|---|---|
| In Press | No |
| Year | 2010 |
| Title | Dendritic chain reaction: responsive release of hydrogen peroxide upon generation and enzymatic oxidation of methanol |
| Authors | Avital-Shmilovici, M; Shabat, D |
| Journal | Bioorganic & Medicinal Chemistry |
| Volume | 18 |
| Issue | 11 |
| Page Numbers | 3643-3647 |
| Abstract | Signal amplification dramatically increases the sensitivity of diagnostic methods. Recently, we introduced a new technique for signal amplification that uses a distinctive dendritic chain reaction (DCR) to generate exponential evolution of a diagnostic signal. In this report, we demonstrate how the modular design of our DCR probe can be used to improve the detection sensitivity. We synthesized a new probe based on a methyl carbonate linkage, which has superior stability in aqueous media. Triggered release of methanol, which was oxidized by alcohol oxidase present in the solution, produced hydrogen peroxide that used as a reagent in the DCR amplification technique. The new probe exhibited higher sensitivity in detection of hydrogen peroxide than our previously reported probe. |
| Doi | 10.1016/j.bmc.2010.02.038 |
| Pmid | 20231097 |
| Wosid | WOS:000278078600002 |
| Is Certified Translation | No |
| Dupe Override | No |
| Comments | Source: Web of Science 000278078600002 |
| Is Public | Yes |
| Language Text | English |
| Keyword | Self-immolative dendrimer; Enzyme; Hydrogen peroxide; Amplification |
| Is Qa | No |