Induction of apoptosis in human acute leukemia Jurkat T cells by Albizzia julibrissin extract is mediated via mitochondria-dependent caspase-3 activation
Won, HJ; Han, CH; Kim, YH; Kwon, HJ; Kim, BW; Choi, JS; Kim, KH
| HERO ID | 1053670 |
|---|---|
| In Press | No |
| Year | 2006 |
| Title | Induction of apoptosis in human acute leukemia Jurkat T cells by Albizzia julibrissin extract is mediated via mitochondria-dependent caspase-3 activation |
| Authors | Won, HJ; Han, CH; Kim, YH; Kwon, HJ; Kim, BW; Choi, JS; Kim, KH |
| Journal | Journal of Ethnopharmacology |
| Volume | 106 |
| Issue | 3 |
| Page Numbers | 383-389 |
| Abstract | To understand antitumor activity of Albizzia julibrissin Durazz (Leguminosae), which has been used as a traditional oriental medicine, the mechanism underlying cytotoxic effect of its extract on human acute leukemia Jurkat T cells were investigated. The methanol extract of the stripped barks (3kg) of Albizzia julibrissin was evaporated, dissolved in water, and then sequentially extracted by chloroform, ethyl acetate, and n-butanol. The substance in the butanol extract containing the most cytotoxic activity was further purified by a series of preparative column chromatography. The active substance obtained (723mg) was designated as HaBC18. When Jurkat T cells were treated with HaBC18 (0.5-2microg/ml), apoptosis along with several biochemical events such as mitochondrial cytochrome c release, activation of caspase-9 and -3, degradation of PARP, and DNA fragmentation was induced in a dose-dependent manner. However, the HaBC18-induced apoptosis was abrogated by an ectopic overexpression of Bcl-xL, which is known to block mitochondrial cytochrome c release. Primary cultures of human PBMC were less sensitive to the cytotoxicity relative to Jurkat T cells. These results demonstrate that the cytotoxicity of HaBC18 toward Jurkat T cells is attributable to apoptosis mediated by mitochondria-dependent death-signaling pathway regulated by Bcl-xL. |
| Doi | 10.1016/j.jep.2006.01.027 |
| Pmid | 16533581 |
| Wosid | WOS:000238965900016 |
| Is Certified Translation | No |
| Dupe Override | No |
| Comments | Source: Web of Science WOS:000238965900016 |
| Is Public | Yes |
| Language Text | English |
| Keyword | Albizzia julibrissin; cytotoxicity; apoptosis; Jurkat T cell; anticancer |
| Is Qa | No |