Problems with multiple use of transfer buffer in protein electrophoretic transfer
Dorri, Y; Kurien, BT; Scofield, RH
HERO ID
1053809
Reference Type
Journal Article
Subtype
Comment
Year
2010
Language
English
PMID
| HERO ID | 1053809 |
|---|---|
| Material Type | Comment |
| In Press | No |
| Year | 2010 |
| Title | Problems with multiple use of transfer buffer in protein electrophoretic transfer |
| Authors | Dorri, Y; Kurien, BT; Scofield, RH |
| Volume | 21 |
| Issue | 1 |
| Page Numbers | 1-2 |
| Abstract | Two-dimensional gel electrophoresis (2DE) and SDS-PAGE are the two most useful methods in protein separation. Proteins separated by 2DE or SDS-PAGE are usually transferred to membranes using a variety of methods, such as electrophoretic transfer, heat-mediated transfer, or nonelectrophoretic transfer, for specific protein detection and/or analysis. In a recent study, Pettegrew et al. claim to reuse transfer buffer containing methanol for at least five times for transferring proteins from SDS-PAGE to polyvinylidene difluoride. They add 150-200 ml fresh transfer solution each time for extended use as a result of loss of transfer buffer. Finally, they test efficiency of each protein transfer by chemiluminescence detection. Here, we comment on this report, as we believe this method is not accurate and useful for protein analysis, and it can cause background binding as well as inaccurate protein analysis. |
| Pmid | 20357975 |
| Is Certified Translation | No |
| Dupe Override | No |
| Comments | Journal: Journal of biomolecular techniques : JBT ISSN: 1943-4731 |
| Is Public | Yes |
| Language Text | English |
| Is Qa | No |