Different properties of electrospun fibrous scaffolds of separated heavy-chain and light-chain fibroins of Bombyx mori
Wadbua, P; Promdonkoy, B; Maensiri, S; Siri, S
| HERO ID | 1053877 |
|---|---|
| In Press | No |
| Year | 2010 |
| Title | Different properties of electrospun fibrous scaffolds of separated heavy-chain and light-chain fibroins of Bombyx mori |
| Authors | Wadbua, P; Promdonkoy, B; Maensiri, S; Siri, S |
| Journal | International Journal of Biological Macromolecules |
| Volume | 46 |
| Issue | 5 |
| Page Numbers | 493-501 |
| Abstract | This study is the first to report on the fabrication and properties of electrospun scaffolds derived from separated light-chain fibroin and heavy-chain fibroin, two major proteins of silk fibroin. Among seven different extraction conditions, which were commonly used to extract fibroin from cocoons of Bombyx mori, only Ajisawa's reagent and 9 M lithium thiocyanate could extract both heavy-chain fibroin and light-chain fibroin, while the other conditions could yield only the light-chain fibroin. Mixed fibroin, light-chain fibroin, and heavy-chain fibroin were fabricated using electrospinning methods. Average diameters of the fibers were 658+/-208, 517+/-162, and 518+/-171 nm, respectively and their sizes after treatment with 50% methanol for 60 min were slightly increased to 747+/-244, 556+/-164 and 521+/-201 nm, respectively. FTIR spectra showed similar predominant beta-sheet conformation of mixed fibroin and heavy-chain fibroin scaffolds after treated with methanol, whereas the predominant structure of light-chain fibroin was random coil conformation. Although, scaffolds derived from mixed fibroin and heavy-chain fibroin showed similar properties, the light-chain fibroin scaffold clearly exhibited different properties, including more hydrophilic character, water uptake ability, degradation rate, and cell adhesion capability. |
| Doi | 10.1016/j.ijbiomac.2010.03.007 |
| Pmid | 20338193 |
| Wosid | WOS:000278586200005 |
| Is Certified Translation | No |
| Dupe Override | No |
| Comments | Source: Web of Science 000278586200005 |
| Is Public | Yes |
| Language Text | English |
| Keyword | Electrospinning; Nanofiber; Light-chain fibroin; Heavy-chain fibroin |
| Is Qa | No |