Dynamic supported liquid membrane tip extraction of glyphosate and aminomethylphosphonic acid followed by capillary electrophoresis with contactless conductivity detection
See, HH; Hauser, PC; Sanagi, MM; Ibrahim, WA
HERO ID
1055235
Reference Type
Journal Article
Year
2010
Language
English
PMID
| HERO ID | 1055235 |
|---|---|
| In Press | No |
| Year | 2010 |
| Title | Dynamic supported liquid membrane tip extraction of glyphosate and aminomethylphosphonic acid followed by capillary electrophoresis with contactless conductivity detection |
| Authors | See, HH; Hauser, PC; Sanagi, MM; Ibrahim, WA |
| Journal | Journal of Chromatography A |
| Volume | 1217 |
| Issue | 37 |
| Page Numbers | 5832-5838 |
| Abstract | A dynamic supported liquid membrane tip extraction (SLMTE) procedure for the effective extraction and preconcentration of glyphosate (GLYP) and its metabolite aminomethylphosphonic acid (AMPA) in water has been investigated. The SLMTE procedure was performed in a semi-automated dynamic mode and demonstrated a greater performance against a static extraction. Several important extraction parameters such as donor phase pH, cationic carrier concentration, type of membrane solvent, type of acceptor stripping phase, agitation and extraction time were comprehensively optimized. A solution of Aliquat-336, a cationic carrier, in dihexyl ether was selected as the supported liquid incorporated into the membrane phase. Quantification of GLYP and AMPA was carried out using capillary electrophoresis with contactless conductivity detection. An electrolyte solution consisting of 12 mM histidine (His), 8 mM 2-(N-morpholino)ethanesulfonic acid (MES), 75 microM cetyltrimethylammonium bromide (CTAB), 3% methanol, pH 6.3, was used as running buffer. Under the optimum extraction conditions, the method showed good linearity in the range of 0.01-200 microg/L (GLYP) and 0.1-400 microg/L (AMPA), acceptable reproducibility (RSD 5-7%, n=5), low limits of detection of 0.005 microg/L for GLYP and 0.06 microg/L for AMPA, and satisfactory relative recoveries (90-94%). Due to the low cost, the SLMTE device was disposed after each run which additionally eliminated the possibility of carry-over between runs. The validated method was tested for the analysis of both analytes in spiked tap water and river water with good success. |
| Doi | 10.1016/j.chroma.2010.07.054 |
| Pmid | 20696433 |
| Wosid | WOS:000282147400013 |
| Is Certified Translation | No |
| Dupe Override | No |
| Comments | Source: Web of Science 000282147400013 |
| Is Public | Yes |
| Language Text | English |
| Keyword | Supported liquid membrane; Glyphosate; AM PA; Capillary electrophoresis; Contactless conductivity detection |
| Is Qa | No |