Gas chromatography–electron-capture detection of urinary methylhippuric acid isomers as biomarkers of environmental exposure to xylene
Buratti, M; Pellegrino, O; Valla, C; Fustinoni, S; Brambilla, G; Colombi, A
HERO ID
1058498
Reference Type
Journal Article
Year
1999
Language
English
PMID
| HERO ID | 1058498 |
|---|---|
| In Press | No |
| Year | 1999 |
| Title | Gas chromatography–electron-capture detection of urinary methylhippuric acid isomers as biomarkers of environmental exposure to xylene |
| Authors | Buratti, M; Pellegrino, O; Valla, C; Fustinoni, S; Brambilla, G; Colombi, A |
| Journal | Journal of Chromatography. Biomedical Applications |
| Volume | 723 |
| Issue | 1-2 |
| Page Numbers | 95-104 |
| Abstract | BIOSIS COPYRIGHT: BIOL ABS. Methylhippuric acid isomers (MHAs), urinary metabolites of xylenes, were determined, after clean-up by C18-SPE and esterification with hexafluoroisopropanol and diisopropylcarbodiimide, by GC with ECD detection, on an SPB-35 capillary column (30 ml 0.32 mm I.D., 0.25 mum film thickness, beta = 320). S-benzyl-mercapturic acid was used for internal standardization. Chromatographic conditions were: oven temperature 162? C, for 14.2 min; ramp by 30? C/min to 190? C, for 3.5 min; ramp by 30? C/min to 250? C, for 4 min; helium flow rate: 1.7 ml/min; detector and injector temperature: 300? C. The sample (1 mul) was injected with a split injection technique (split ratio 5:1). MHA recovery was and gt;95% in the 0.5-20 mumol/l range; the limit of detection was and lt;0.25 mumol/l; day-to-day precision, at 2 mumol/l, was Cv and lt;10%. Urinary MHAs were determined in subjects exposed to different low-level sources of xylenes: (a) tobacco smoking habit and (b) BTX urban air pollution (airbor |
| Doi | 10.1016/s0378-4347(98)00482-4 |
| Pmid | 10080637 |
| Wosid | WOS:000078700800011 |
| Url | http://linkinghub.elsevier.com/retrieve/pii/S0378434798004824 |
| Is Certified Translation | No |
| Dupe Override | No |
| Is Public | Yes |
| Language Text | English |
| Keyword | xylene; methylhippuric acid |
| Is Qa | No |