Quantitative determination of spinosin in rat plasma by liquid chromatography-tandem mass spectrometry method
Li, Y; Yao, M; Cheng, S
| HERO ID | 1071006 |
|---|---|
| In Press | No |
| Year | 2008 |
| Title | Quantitative determination of spinosin in rat plasma by liquid chromatography-tandem mass spectrometry method |
| Authors | Li, Y; Yao, M; Cheng, S |
| Journal | Journal of Pharmaceutical and Biomedical Analysis |
| Volume | 48 |
| Issue | 4 |
| Page Numbers | 1169-1173 |
| Abstract | A sensitive method for the quantitative determination of spinosin in rat plasma was developed and validated using high-performance liquid chromatographic separation with tandem mass spectrometric detection. The analytes of interest were extracted from rat plasma samples by methyl tert-butyl ether (MTBE) after acidification with 1.0% acetic acid aqueous solution. Chromatographic separation was achieved on an Agilent Zorbax SB-C(18) (50 mm x 4.6 mm, 5 microm) using a isocratic mobile phase consisting of acetonitrile-water (30:70, v/v) with 1% isopropyl alcohol and 0.01% heptafluorobutyric acid. The flow rate was 0.2 ml/min. The column temperature was maintained at 25 degrees C. Detection was performed on a triple quadrupole tandem mass spectrometer by selected reaction monitoring (SRM) mode via electrospray ionization (ESI). The calibration curve was linear over the range of 1.00-400 ng/ml in rat plasma, with 1.00 ng/ml of the lower limit of quantification (LLOQ). The inter- and intra-day precisions and accuracy for all samples were satisfactory. The validated method was successfully applied for the pharmacokinetic study of spinosin in rat. After oral administration of 20mg/kg spinosin to rats, the main pharmacokinetic parameters of T(max), C(max), T(0.5) and AUC(0-T) were 5.33+/-0.58 h, 132.2+/-10.6 ng/ml, 4.89+/-0.37 h, 1.02+/-0.09 microg h/l, respectively. |
| Doi | 10.1016/j.jpba.2008.08.025 |
| Pmid | 18834689 |
| Wosid | WOS:000262622000016 |
| Is Certified Translation | No |
| Dupe Override | No |
| Comments | Source: Web of Science WOS:000262622000016Scopus URL: https://www.scopus.com/inward/record.uri?eid=2-s2.0-57849166190&doi=10.1016%2fj.jpba.2008.08.025&partnerID=40&md5=a99679f0016c88c88e4922f523161eaa |
| Is Public | Yes |
| Language Text | English |
| Keyword | Spinosin; Rat plasma; Pharmacokinetics; LC-MS |
| Is Qa | No |