Particulate air pollution induces arrhythmia via oxidative stress and calcium calmodulin kinase II activation
Kim, JB; Kim, C; Choi, E; Park, S; Park, H; Pak, HN; Lee, MH; Shin, DC; Hwang, KC; Joung, B
HERO ID
1073525
Reference Type
Journal Article
Year
2012
Language
English
PMID
| HERO ID | 1073525 |
|---|---|
| In Press | No |
| Year | 2012 |
| Title | Particulate air pollution induces arrhythmia via oxidative stress and calcium calmodulin kinase II activation |
| Authors | Kim, JB; Kim, C; Choi, E; Park, S; Park, H; Pak, HN; Lee, MH; Shin, DC; Hwang, KC; Joung, B |
| Journal | Toxicology and Applied Pharmacology |
| Volume | 259 |
| Issue | 1 |
| Page Numbers | 66-73 |
| Abstract | Ambient particulate matter (PM) can increase the incidence of arrhythmia. However, the arrhythmogenic mechanism of PM is poorly understood. This study investigated the arrhythmogenic mechanism of PM. In Sprague-Dawley rats, QT interval was increased from 115.0±14.0 to 142.1±18.4ms (p=0.02) after endotracheal exposure of DEP (200μg/ml for 30min, n=5). Ventricular premature contractions were more frequently observed after DEP exposure (100%) than baseline (20%, p=0.04). These effects were prevented by pretreatment of N-acetylcysteine (NAC, 5mmol/L, n=3). In 12 Langendorff-perfused rat hearts, DEP infusion of 12.5μg/ml for 20min prolonged action potential duration (APD) at only left ventricular base increasing apicobasal repolarization gradients. Spontaneous early afterdepolarization (EAD) and ventricular tachycardia (VT) were observed in 8 (67%) and 6 (50%) hearts, respectively, versus no spontaneous triggered activity or VT in any hearts before DEP infusion. DEP-induced APD prolongation, EAD and VT were successfully prevented with NAC (5mmol/L, n=5), nifedipine (10μmol/L, n=5), and active Ca(2+)/calmodulin-dependent protein kinase II (CaMKII) blockade, KN 93 (1μmol/L, n=5), but not by thapsigargin (200nmol/L) plus ryanodine (10μmol/L, n=5) and inactive CaMKII blockade, KN 92 (1μmol/L, n=5). In neonatal rat cardiomyocytes, DEP provoked ROS generation in dose dependant manner. DEP (12.5μg/ml) induced apoptosis, and this effect was prevented by NAC and KN 93. Thus, this study shows that in vivo and vitro exposure of PM induced APD prolongation, EAD and ventricular arrhythmia. These effects might be caused by oxidative stress and CaMKII activation. |
| Doi | 10.1016/j.taap.2011.12.007 |
| Pmid | 22197715 |
| Wosid | WOS:000301094800007 |
| Is Certified Translation | No |
| Dupe Override | No |
| Comments | Source: Web of Science 000301094800007 |
| Is Public | Yes |
| Language Text | English |
| Keyword | Particulate matter; Oxidative stress; Ca2+/calmodulin-dependent protein kinase II; Ventricular tachycardia; Ventricular fibrillation |
| Is Qa | No |