Influence of fluoro, chloro and alkyl alcohols on the folding pathway of human serum albumin
Kumar, Y; Muzammilt, S; Tayyab, S
| HERO ID | 1150412 |
|---|---|
| In Press | No |
| Year | 2005 |
| Title | Influence of fluoro, chloro and alkyl alcohols on the folding pathway of human serum albumin |
| Authors | Kumar, Y; Muzammilt, S; Tayyab, S |
| Journal | Journal of Biochemistry |
| Volume | 138 |
| Issue | 4 |
| Page Numbers | 335-341 |
| Abstract | Urea-induced equilibrium unfolding of human serum albumin (HSA) when studied by mean residue ellipticity at 222 nm (MRE(222)) or intrinsic fluorescence measurements showed a two-step, three-state transition with a stable intermediate around 4.6-5.2 M urea. The presence of 2,2,2-trifluoroethanol (TFE) resulted in a single-step, two-state transition with a significant shift towards higher urea concentration, suggesting the stabilizing effect of TFE. The free energy of stabilization (DeltaDeltaG(D)(H(2)O)) in the presence of 3.0 M TFE was determined to be 2.68 and 2.72 kcal/mol by MRE(222) and fluorescence measurements, respectively. The stabilizing potential of other alcohols on the refolding behavior of HSA at 5.0 M urea (where the intermediate exists) as studied by MRE(222) and intrinsic fluorescence measurements showed the following order: 1,1,1,3,3,3-hexafluoroisopropanol (HFIP) > TFE > 2-chloroethanol > tert-butanol > iso-propanol > ethanol > methanol. Further, the extent of refolding at the highest concentration of alcohol was similar in all cases. The stabilizing effect of TFE on guanidine hydrochloride (GdnHCl)-induced unfolding of HSA was nearly equal to that found for urea denaturation, as reflected in the DeltaDeltaG(D)(H(2)O) value (2.38 kcal/mol). Taken together, these results suggest that the stabilizing effect of TFE and other alcohols on urea/GdnHCl-induced unfolding of HSA is higher for alcohols that contain bulky groups or fluorine atoms. |
| Doi | 10.1093/jb/mvi131 |
| Pmid | 16272127 |
| Wosid | WOS:000233244300001 |
| Is Certified Translation | No |
| Dupe Override | No |
| Comments | Source: Web of Science 000233244300001 |
| Is Public | Yes |
| Language Text | English |
| Keyword | alcohols; circular dichroism; fluorescence; folding pathway; human serum albumin; trifluoroethanol; urea denaturation |
| Is Qa | No |