Determination of tetrabromobisphenol A in human serum by liquid chromatography-electrospray ionization tandem mass spectrometry
Hayama, T; Yoshida, H; Onimaru, S; Yonekura, S; Kuroki, H; Todoroki, K; Nohta, H; Yamaguchi, M
HERO ID
1155600
Reference Type
Journal Article
Year
2004
Language
English
PMID
| HERO ID | 1155600 |
|---|---|
| In Press | No |
| Year | 2004 |
| Title | Determination of tetrabromobisphenol A in human serum by liquid chromatography-electrospray ionization tandem mass spectrometry |
| Authors | Hayama, T; Yoshida, H; Onimaru, S; Yonekura, S; Kuroki, H; Todoroki, K; Nohta, H; Yamaguchi, M |
| Journal | Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences |
| Volume | 809 |
| Issue | 1 |
| Page Numbers | 131-136 |
| Abstract | A method for the determination of tetrabromobisphenol A (TBBPA) in human serum utilizing solid-phase extractions (SPEs) and liquid chromatography (LC) with electrospray ionization tandem MS (MS/MS) has been developed. After purification and concentration of TBBPA using consecutive SPEs on reversed-phase and normal-phase cartridges, the serum sample was subjected to LC. TBBPA was separated on a C18 reversed-phase column by gradient elution with a mixture of water, methanol, and acetonitrile as the mobile phase, and then detected with electrospray ionization MS/MS in negative ion mode. 13C12-TBBPA was suitable as an internal standard for the reproducible determination of TBBPA in human serum samples (5 g). The method has been validated in TBBPA concentration range of 5-100 pg per g serum, and the recoveries in the concentration range were higher than 83.3%. The repeatabilities of the proposed method of non-spiked control serum (6.3 pg per g serum) and spiked serum (added 5-100 pg per g serum) were within 10.0% as relative standard deviations. The limit of quantification (LOQ) for TBBPA was 4.1 pg per g serum, which was corresponded to 0.63 fmol on column. |
| Doi | 10.1016/j.jchromb.2004.06.013 |
| Pmid | 15282103 |
| Wosid | WOS:000223240500019 |
| Url | http://www.sciencedirect.com/science/article/pii/S1570023204004957 |
| Is Certified Translation | No |
| Dupe Override | No |
| Comments | Scopus URL: https://www.scopus.com/inward/record.uri?eid=2-s2.0-84859509521&doi=10.3724%2fSP.J.1123.2011.01165&partnerID=40&md5=183ffdb977095d90ca30b26e8abd6ff1 |
| Is Public | Yes |
| Language Text | English |
| Keyword | tetrabisphenol A |
| Relationship(s) |
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