Determination of 5-hydroxy-N-methylpyrrolidone and 2-hydroxy-N-methylsuccinimide in human urine
Jonsson, BAG; Akesson, B
| HERO ID | 1186716 |
|---|---|
| In Press | No |
| Year | 1997 |
| Title | Determination of 5-hydroxy-N-methylpyrrolidone and 2-hydroxy-N-methylsuccinimide in human urine |
| Authors | Jonsson, BAG; Akesson, B |
| Journal | Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences |
| Volume | 694 |
| Issue | 2 |
| Page Numbers | 351-357 |
| Abstract | A method for simultaneous determination of 5-hydroxy-N-methylpyrrolidone and 2-hydroxy-N-methylsuccinimide in urine is described. These compounds are metabolites of N-methyl-2-pyrrolidone, a powerful and widely used organic solvent. 5-Hydroxy-N-methylpyrrolidone and 2-hydroxy-N-methylsuccinimide were purified from urine by adsorption to a C8 solid-phase extraction column and then elution by ethyl acetate-methanol (80:20). After evaporation, the samples were derivatised at 100 degrees C for 1 h by bis(trimethylsilyl)trifluoroacetamide. Ethyl acetate was then added and the samples were analysed by gas chromatography-mass spectrometry in the electron impact mode. The extraction recovery for 5-hydroxy-N-methylpyrrolidone was about 80% while that for 2-hydroxy-N-methylsuccinimide was about 30%. The intra-day precision for 5-hydroxy-N-methylpyrrolidone was 2-4% and the between-day precision 4-21% (4 and 60 microg/ml). The intra-day precision for 2-hydroxy-N-methylsuccinimide was 4-8% and the between-day precision 6-7% (2 and 20 microg/ml). The detection limit was 0.2 microg/ml urine for both compounds. The method is applicable for analysis of urine samples from workers exposed to N-methyl-2-pyrrolidone. |
| Doi | 10.1016/S0378-4347(97)00125-4 |
| Pmid | 9252049 |
| Wosid | WOS:A1997XL73200011 |
| Is Certified Translation | No |
| Dupe Override | No |
| Comments | Source: Web of Science A1997XL73200011 |
| Is Public | Yes |
| Language Text | English |
| Keyword | 5-hydroxy-N-methylpyrrolidone; 2-hydroxy-N-methylsuccininide; N-methyl-2-pyrrolidone |
| Is Qa | No |