Novel monolithic poly(p-methylstyrene-co-bis(p-vinylbenzyl)dimethylsilane) capillary columns for biopolymer separation
Wieder, W; Lubbad, SH; Trojer, L; Bisjak, CP; Bonn, GK
HERO ID
1253380
Reference Type
Journal Article
Year
2008
Language
English
PMID
| HERO ID | 1253380 |
|---|---|
| In Press | No |
| Year | 2008 |
| Title | Novel monolithic poly(p-methylstyrene-co-bis(p-vinylbenzyl)dimethylsilane) capillary columns for biopolymer separation |
| Authors | Wieder, W; Lubbad, SH; Trojer, L; Bisjak, CP; Bonn, GK |
| Journal | Journal of Chromatography A |
| Volume | 1191 |
| Issue | 1-2 |
| Page Numbers | 253-262 |
| Abstract | Hydrophobic organo-silane based monolithic capillary columns were prepared by thermally initiated free radical polymerisation within the confines of 200 microm i.d. fused silica capillaries. A novel crosslinker, namely bis(p-vinylbenzyl)dimethylsilane (BVBDMS), was copolymerised with p-methylstyrene (MS) in the presence of 2-propanol and toluene, using alpha,alpha'-azoisobutyronitrile (AIBN) as initiator. Monolithic capillary columns, differing in the total monomer, microporogen content and microporogen nature were fabricated and the chromatographic efficiency of each monolith, regarding the separation of proteins, peptides and oligonucleotides, was evaluated and compared. Changes in monolith morphology were monitored by scanning electron microscopy (SEM). Porosity and specific surface areas of the supports were studied by means of mercury intrusion porosimetry and BET measurements, respectively. Pressure drop vs. flow rate measurements proved the prepared poly(p-methylstyrene-co-bis(p-vinylbenzyl)dimethylsilane) (MS/BVBDMS) monoliths to be mechanically stable and swelling propensity (SP) factors of 0.78-1.10 indicate high crosslinking homogeneity. |
| Doi | 10.1016/j.chroma.2007.11.070 |
| Pmid | 18093606 |
| Is Certified Translation | No |
| Dupe Override | No |
| Is Public | Yes |
| Language Text | English |
| Is Qa | No |