A marine sulfate-reducing bacterium producing multiple antibiotics: biological and chemical investigation

Zhang, Y; Mu, J; Gu, X; Zhao, C; Wang, X; Xie, Z

HERO ID

1323146

Reference Type

Journal Article

Year

2009

Language

English

PMID

19841718

HERO ID 1323146
In Press No
Year 2009
Title A marine sulfate-reducing bacterium producing multiple antibiotics: biological and chemical investigation
Authors Zhang, Y; Mu, J; Gu, X; Zhao, C; Wang, X; Xie, Z
Journal Marine Drugs
Volume 7
Issue 3
Page Numbers 341-354
Abstract A marine sulfate-reducing bacterium SRB-22 was isolated by means of the agar shake dilution method and identified as Desulfovibrio desulfuricans by morphological, physiological and biochemical characteristics and 16S rDNA analysis. In the bioassay, its extract showed broad-spectrum antimicrobial activity using the paper disc agar diffusion method. This isolate showed a different antimicrobial profile than either ampicillin or nystatin and was found to produce at least eight antimicrobial components by bioautography. Suitable fermentation conditions for production of the active constituents were determined to be 28 day cultivation at 25 degrees C to 30 degrees C with a 10% inoculation ratio. Under these conditions, the SRB-22 was fermented, extracted and chemically investigated. So far an antimicrobial compound, mono-n-butyl phthalate, and an inactive compound, thymine, have been isolated and characterized.
Doi 10.3390/md7030341
Pmid 19841718
Wosid WOS:000270196400004
Url https://search.proquest.com/docview/1536059173?accountid=171501
Is Certified Translation No
Dupe Override No
Is Public Yes
Language Text English
Keyword marine sulfate-reducing bacterium; isolation; identification; broad-spectrum antimicrobial activity; mono-n-butyl phthalate