Masking effect of anti-androgens on androgenic activity in European river sediment unveiled by effect-directed analysis
Weiss, JM; Hamers, T; Thomas, KV; van der Linden, S; Leonards, PE; Lamoree, MH
HERO ID
1323154
Reference Type
Journal Article
Year
2009
Language
English
PMID
| HERO ID | 1323154 |
|---|---|
| In Press | No |
| Year | 2009 |
| Title | Masking effect of anti-androgens on androgenic activity in European river sediment unveiled by effect-directed analysis |
| Authors | Weiss, JM; Hamers, T; Thomas, KV; van der Linden, S; Leonards, PE; Lamoree, MH |
| Journal | Analytical and Bioanalytical Chemistry |
| Volume | 394 |
| Issue | 5 |
| Page Numbers | 1385-1397 |
| Abstract | This study shows that the androgen receptor agonistic potency is clearly concealed by the effects of androgen receptor antagonists in a total sediment extract, demonstrating that toxicity screening of total extracts is not enough to evaluate the full in vitro endocrine disrupting potential of a complex chemical mixture, as encountered in the environment. The anti-androgenic compounds were masking the activity of androgenic compounds in the extract with relatively high anti-androgenic potency, equivalent to 200 nmol flutamide equivalents/g dry weight. A two-step serial liquid chromatography fractionation of the extract successfully separated anti-androgenic compounds from androgenic compounds, resulting in a total androgenic potency of 3,820 pmol dihydrotestosterone equivalents/g dry weight. The fractionation simplified the chemical identification analysis of the original complex sample matrix. Seventeen chemical structures were tentatively identified. Polyaromatic hydrocarbons, a technical mixture of nonylphenol and dibutyl phthalate were identified to contribute to the anti-androgenic potency observed in the river sediment sample. With the GC/MS screening method applied here, no compounds with AR agonistic disrupting potencies could be identified. Seventy-one unidentified peaks, which represent potentially new endocrine disrupters, have been added to a database for future investigation. |
| Doi | 10.1007/s00216-009-2807-8 |
| Pmid | 19418041 |
| Wosid | WOS:000266918600018 |
| Url | https://search.proquest.com/docview/46307269?accountid=171501 |
| Is Certified Translation | No |
| Dupe Override | No |
| Comments | Source: Web of Science WOS:000266918600018 |
| Is Public | Yes |
| Language Text | English |
| Keyword | Androgenicity; Androgen receptor (AR); Effect-directed analysis (EDA); Sediment; Polycyclic aromatic hydrocarbons (PAHs); MODELKEY |