Cloning of a dibutyl phthalate hydrolase gene from Acinetobacter sp. strain M673 and functional analysis of its expression product in Escherichia coli
Wu, J; Liao, X; Yu, F; Wei, Z; Yang, L
HERO ID
1332542
Reference Type
Journal Article
Year
2013
Language
English
PMID
| HERO ID | 1332542 |
|---|---|
| In Press | No |
| Year | 2013 |
| Title | Cloning of a dibutyl phthalate hydrolase gene from Acinetobacter sp. strain M673 and functional analysis of its expression product in Escherichia coli |
| Authors | Wu, J; Liao, X; Yu, F; Wei, Z; Yang, L |
| Journal | Applied Microbiology and Biotechnology |
| Volume | 97 |
| Issue | 6 |
| Page Numbers | 2483-2491 |
| Abstract | A dibutyl phthalate (DBP) transforming bacterium, strain M673, was isolated and identified as Acinetobacter sp. This strain could not grow on dialkyl phthalates, including dimethyl, diethyl, dipropyl, dibutyl, dipentyl, dihexyl, di(2-ethylhexyl), di-n-octyl, and dinonyl phthalate, but suspensions of cells could transform these compounds to phthalate via corresponding monoalkyl phthalates. During growth in Luria-Bertani medium, M673 produced the high amounts of non-DBP-induced intracellular hydrolase in the stationary phase. One DBP hydrolase gene containing an open reading frame of 1,095 bp was screened from a genomic library, and its expression product hydrolyzed various dialkyl phthalates to the corresponding monoalkyl phthalates. |
| Doi | 10.1007/s00253-012-4232-8 |
| Pmid | 22729233 |
| Wosid | WOS:000315650100018 |
| Url | https://search.proquest.com/docview/1469194629?accountid=171501 |
| Is Certified Translation | No |
| Dupe Override | No |
| Is Public | Yes |
| Language Text | English |
| Keyword | article; Acinetobacter; Carboxylic Ester Hydrolases; Cloning, Molecular; DNA, Bacterial; Dibutyl Phthalate; Escherichia coli; Kinetics; Molecular Sequence Data; Sequence Analysis, DNA; Substrate Specificity; bacteria; cell suspension culture; genomic libraries; open reading frames |