The lung tumor promoter, butylated hydroxytoluene (BHT), causes chronic inflammation in promotion-sensitive BALB/cByJ mice but not in promotion-resistant CXB4 mice
Bauer, AK; Dwyer-Nield, LD; Hankin, JA; Murphy, RC; Malkinson, AM
HERO ID
1419575
Reference Type
Journal Article
Year
2001
Language
English
PMID
| HERO ID | 1419575 |
|---|---|
| In Press | No |
| Year | 2001 |
| Title | The lung tumor promoter, butylated hydroxytoluene (BHT), causes chronic inflammation in promotion-sensitive BALB/cByJ mice but not in promotion-resistant CXB4 mice |
| Authors | Bauer, AK; Dwyer-Nield, LD; Hankin, JA; Murphy, RC; Malkinson, AM |
| Journal | Toxicology |
| Volume | 169 |
| Issue | 1 |
| Page Numbers | 1-15 |
| Abstract | An inflammatory response accompanies the reversible pneumotoxicity caused by butylated hydroxytoluene (BHT) administration to mice. Lung tumor formation is promoted by BHT administration following an initiating agent in BALB/cByJ mice, but not in CXB4 mice. To assess the contribution of inflammation to this differential susceptibility, we quantitatively characterized inflammation after one 150 mg/kg body weight, followed by three weekly 200 mg/kg ip injections of BHT into male mice of both strains. This examination included inflammatory cell infiltrate and protein contents in bronchoalveolar lavage (BAL) fluid, cyclooxygenase (COX)-1 and COX-2 expression in lung extracts, and PGE(2) and PGI(2) production by isolated bronchiolar Clara cells. BAL macrophage and lymphocyte numbers increased in BALB mice (P<0.0007 and 0.02, respectively), as did BAL protein content (P<0.05), COX-1 and COX-2 expression (P<0.05 for each), and PGI(2) production (P<0.05); conversely, these indices were not perturbed by BHT in CXB4 mice. BALB mice fed aspirin (400 mg/kg of chow) for two weeks prior to BHT treatment had reduced inflammatory cell infiltration. Our results support a hypothesis that resistance to BHT-induced inflammation in CXB4 mice accounts, at least in part, for the lack of effect of BHT on lung tumor multiplicity in this strain. |
| Doi | 10.1016/S0300-483X(01)00475-9 |
| Pmid | 11696405 |
| Wosid | WOS:000172613500001 |
| Is Certified Translation | No |
| Dupe Override | No |
| Comments | Scopus URL: https://www.scopus.com/inward/record.uri?eid=2-s2.0-0035499381&doi=10.1016%2fS0300-483X%2801%2900475-9&partnerID=40&md5=e415d04b4c3d7e45139103f05ef7c7ae |
| Is Public | Yes |
| Language Text | English |
| Keyword | chronic inflammation; strain differences; bronchoalveolar lavage; cyclooxygenase; prostaglandins |