Determination of clenbuterol in urine by azo-dye precolumn derivatization and micellar liquid chromatography
Biosca, YM; Baeza, JJB; Ramisramos, G
| HERO ID | 1455593 |
|---|---|
| In Press | No |
| Year | 1997 |
| Title | Determination of clenbuterol in urine by azo-dye precolumn derivatization and micellar liquid chromatography |
| Authors | Biosca, YM; Baeza, JJB; Ramisramos, G |
| Journal | Chromatographia |
| Volume | 44 |
| Issue | 3-4 |
| Page Numbers | 145-150 |
| Abstract | Clenbuterol has been determined in urine by solid-phase extraction on a C-18 cartridge, diazotization of the eluate with nitrite, coupling of the diazonium ion with 1-(naphthyl)ethylenediamine, and separation of the azo dye formed by HPLC with a C-18 column and a micellar mobile phase containing 0.1 M sodium dodecyl sulphate, 12% n-butanol and 0.05 M citrate buffer, pH 3. Recoveries higher than 90% were obtained by mixing the samples with a 20% 0.2 M NaOH before extraction. Limits of detection of 51 and 6.7 ng L(-1) were obtained with spectrophotometric and thermal lens spectrometric detection, respectively; respective repeatabilities were 3.1% (5 mu g mL(-1)) and 5.6% (0.16 mu g mL(-1)). |
| Wosid | WOS:A1997WN63400005 |
| Is Certified Translation | No |
| Dupe Override | No |
| Comments | Source: Web of Science WOS:A1997WN63400005 |
| Is Public | Yes |
| Keyword | column liquid chromatography; thermal lens spectrometric detection; diazotization and coupling derivatization; clenbuterol in urine; N-(naphthyl)ethylenediamine |