Chicory increases acetate turnover, but not propionate and butyrate peripheral turnovers in rats
Pouteau, E; Rochat, F; Jann, A; Meirim, I; Sanchez-Garcia, JL; Ornstein, K; German, B; Ballevre, O
| HERO ID | 1456234 |
|---|---|
| In Press | No |
| Year | 2008 |
| Title | Chicory increases acetate turnover, but not propionate and butyrate peripheral turnovers in rats |
| Authors | Pouteau, E; Rochat, F; Jann, A; Meirim, I; Sanchez-Garcia, JL; Ornstein, K; German, B; Ballevre, O |
| Journal | British Journal of Nutrition |
| Volume | 99 |
| Issue | 2 |
| Page Numbers | 287-296 |
| Abstract | Chicory roots are rich in inulin that is degraded into SCFA in the caecum and colon. Whole-body SCFA metabolism was investigated in rats during food deprivation and postprandial states. After 22 h of food deprivation, sixteen rats received an IV injection of radioactive 14C-labelled SCFA. The volume of distribution and the fractional clearance rate of SCFA were 0.25-0.27 litres/kg and 5.4-5.9 %/min, respectively. The half-life in the first extracellular rapidly decaying compartment was between 0.9 and 1.4 min. After 22 h of food deprivation, another seventeen rats received a primed continuous IV infusion of 13C-labelled SCFA for 2 h. Isotope enrichment (13C) of SCFA was determined in peripheral arterial blood by MS. Peripheral acetate, propionate and butyrate turnover rates were 29, 4 and 0.3 micromol/kg per min respectively. Following 4 weeks of treatment with chicory root or control diets, eighteen fed rats received a primed continuous IV infusion of 13C-labelled SCFA for 2 h. Intestinal degradation of dietary chicory lowered caecal pH, enhanced caecal and colonic weights, caecal SCFA concentrations and breath H2. The diet with chicory supplementation enhanced peripheral acetate turnover by 25 % (P = 0.017) concomitant with an increase in plasma acetate concentration. There were no changes in propionate or butyrate turnovers. In conclusion, by setting up a multi-tracer approach to simultaneously assess the turnovers of acetate, propionate and butyrate it was demonstrated that a chronic chicory-rich diet significantly increases peripheral acetate turnover but not that of propionate or butyrate in rats. |
| Doi | 10.1017/S0007114507815790 |
| Pmid | 17761014 |
| Wosid | WOS:000253045600012 |
| Is Certified Translation | No |
| Dupe Override | No |
| Comments | Source: Web of Science WOS:000253045600012 |
| Is Public | Yes |
| Language Text | English |
| Keyword | short-chain fatty acids; chicory; kinetics; rats |