Efficacy, safety and immunological actions of butanol-extracted Food Allergy Herbal Formula-2 on peanut anaphylaxis
Srivastava, K; Yang, N; Chen, Y; Lopez-Exposito, I; Song, Y; Goldfarb, J; Zhan, J; Sampson, H; Li, XM
| HERO ID | 1456376 |
|---|---|
| In Press | No |
| Year | 2011 |
| Title | Efficacy, safety and immunological actions of butanol-extracted Food Allergy Herbal Formula-2 on peanut anaphylaxis |
| Authors | Srivastava, K; Yang, N; Chen, Y; Lopez-Exposito, I; Song, Y; Goldfarb, J; Zhan, J; Sampson, H; Li, XM |
| Journal | Clinical and Experimental Allergy |
| Volume | 41 |
| Issue | 4 |
| Page Numbers | 582-591 |
| Abstract | <strong>BACKGROUND: </strong>Therapies for peanut allergy (PNA) are urgently needed. Food Allergy Herbal Formula-2 (FAHF-2) has profound therapeutic effects in a murine PNA model and is safe for food-allergic adults in clinical trials. However, the large FAHF-2 pill-load is not conducive to clinical studies in children. Thus, refining FAHF-2 to decrease pill-load is essential for the inclusion of children in clinical trials and to facilitate studying FAHF-2 as a clinically useful botanical drug.<br /><br /><strong>OBJECTIVES: </strong>Testing long-term efficacy and safety of a butanol-purified extract of FAHF-2 (B-FAHF-2) in a murine model of PNA, and to explore its immunological mechanisms of action.<br /><br /><strong>METHODS: </strong>FAHF-2 was purified by butanol extraction. C3H/HeJ mice with established PNA received the first course of B-FAHF-2 at 6 mg, twice daily for 7 weeks (PNA/B-FAHF-2) or water (PNA/sham) and were then challenged immediately after completing the treatment and six more times every 1-2 months post-treatment up to week 50. Mice then received a second course of B-FAHF-2 treatment at week 52 and were challenged at week 65. In vivo and in vitro immunological effects on T, B and mast cells were also determined.<br /><br /><strong>RESULTS: </strong>Butanol purification reduced the volume of the effective dose ∼5-fold. All PNA/B-FAHF-2 mice were completely protected from PN anaphylaxis until the fifth challenge after the first course of treatment, as compared with PNA/sham mice. Partial protection persisted up to 50 weeks. A second treatment course restored complete protection. B-FAHF-2 significantly suppressed Th2 cytokine, IgE and histamine levels in vivo, and showed direct inhibition of Th2, IgE-producing B cells and mast cell activation in vitro. B-FAHF-2 had a high margin of safety.<br /><br /><strong>CONCLUSION AND CLINICAL RELEVANCE: </strong>B-FAHF-2 produced long-lasting protection against PN anaphylaxis for approximately half of the murine life span without side-effects. B-FAHF-2 exhibited direct effects on multiple food allergy effector cells. |
| Doi | 10.1111/j.1365-2222.2010.03643.x |
| Pmid | 21121976 |
| Wosid | WOS:000288387200017 |
| Is Certified Translation | No |
| Dupe Override | No |
| Comments | Source: Web of Science WOS:000288387200017 |
| Is Public | Yes |
| Language Text | English |
| Keyword | B-FAHF-2; Chinese herbal medicine formula; FAHF-2; histamine; IgE; peanut anaphylaxis; Th2 cytokines |