Subchronic Toxic Effects And Accumulation Of Hexahydro-1,3,5-Trinitro-1,3,5-Triazine (RDX) In Zebrafish (Danio Rerio)
Mukhi, S; Patino, R
| HERO ID | 1466498 |
|---|---|
| In Press | No |
| Year | 2006 |
| Title | Subchronic Toxic Effects And Accumulation Of Hexahydro-1,3,5-Trinitro-1,3,5-Triazine (RDX) In Zebrafish (Danio Rerio) |
| Authors | Mukhi, S; Patino, R |
| Journal | Toxicological Sciences |
| Volume | 90 |
| Issue | 1-S |
| Abstract | The objective of this study was to assess the subchronic (subacute) toxicity of RDX to adult zebrafish. Mixed-sex populations of 3-month-old, young adult fish were exposed to measured RDX concentrations of 0, 1 and 9.6 mg/L for up to 12 weeks followed by a 15-day recovery in clean water. At 9.6 mg/L, RDX induced a cumulative mortality rate of about 45 percent over the 12-week exposure, with most mortalities occurring within the first 4 weeks and becoming negligible after 8 weeks. Fish held at 9.6 mg/L also showed abnormal feeding behavior during the first 8 weeks of exposure; and somatic measurements indicated that RDX at 9.6 mg/L suppressed body weight at 4 and 8 weeks of exposure and at 1 mg/L, it suppressed body weight at 4 weeks. However, the effects of RDX at 9.6 mg/L on feeding behavior were no longer evident after 8 weeks of exposure, and these fish regained normal weight by the end of the 12-week exposure period relative to control fish. Histopathological analyses of gonads from fish collected at 12 weeks of exposure did not reveal structural abnormalities. In males, Comet assay of dispersed testicular cells taken at the completion of the exposure period did not indicate the presence of DNA strand breakages related to RDX exposure. RDX and its metabolite, MNX, were detected at 4, 8, and 12 weeks of RDX exposure. The bioconcentration factor for RDX was affected by time of exposure but not by RDX concentration; average combined values were <= 1 at 4 and 8 weeks of exposure and 2 at 12 weeks. RDX and MNX were not detected in fish after a recovery period of 15 days in clean water. The latter observation suggests that RDX and MNX are readily eliminated from the fish. |
| Is Certified Translation | No |
| Dupe Override | 1466498 |
| Is Public | Yes |
| Language Text | eng |