Identification of alkaloids; the condensation products of biogenic amines with formaldehyde, enzymatically formed from 5-methyltetrahydrofolic acid
Lauwers, W; Leysen, J; Verhoeven, H; Laduron, P
| HERO ID | 1514087 |
|---|---|
| In Press | No |
| Year | 1975 |
| Title | Identification of alkaloids; the condensation products of biogenic amines with formaldehyde, enzymatically formed from 5-methyltetrahydrofolic acid |
| Authors | Lauwers, W; Leysen, J; Verhoeven, H; Laduron, P |
| Journal | Biomedical Mass Spectrometry |
| Volume | 2 |
| Issue | 1 |
| Page Numbers | 15-22 |
| Abstract | The use of thin-layer chromatography has demonstrated that incubations of indoleamines with 5-methyl[14-C]tetrahydrofolic acid and an enzyme previously described as an N-methyltransferase, do not yield Nw, N1, or O-methylated products. Further elucidation by thin-layer chromatography, gas chromatography, mass spectrometry and selected ion monitoring enabled us to identify the reaction products as tetrahydroisoquinolines and tetrahydro-beta-carbolines in mixtures incubated respectively with catecholamines and indoleamines in the presence of 5-methyl[14-C]tetrahydrofolic acid and enzyme. The alkaloids have been shown to originate from a spontaneous condensation of the corresponding amines with formaldehyde, this latter being formed in the first stage of the reaction by enzymatic conversion from 5-methyltetrahydrofolic acid. |
| Pmid | 1131388 |
| Is Certified Translation | No |
| Dupe Override | No |
| Is Public | Yes |
| Language Text | English |
| Keyword | Alkaloids/ chemical synthesis; Animals; Biogenic Amines; Brain/enzymology; Carbolines/chemical synthesis; Chemical Phenomena; Chemistry; Chromatography, Gas; Chromatography, Thin Layer; Folic Acid/ analogs & derivatives; Formaldehyde; Isoquinolines/chemical synthesis; Kidney/enzymology; Mass Spectrometry; Methyltransferases |