BIOLUMINESCENT ASSAY FOR NITRIC OXIDE UTILIZING THE BIOLOGICAL ENZYME ACTIVITY OF SOLUBLE GUANYLATE CYCLASE
Sano, Y; Seki, M; Abe, S; Suzuki, S; Arakawa, H
| HERO ID | 1565924 |
|---|---|
| In Press | No |
| Year | 2011 |
| Title | BIOLUMINESCENT ASSAY FOR NITRIC OXIDE UTILIZING THE BIOLOGICAL ENZYME ACTIVITY OF SOLUBLE GUANYLATE CYCLASE |
| Authors | Sano, Y; Seki, M; Abe, S; Suzuki, S; Arakawa, H |
| Journal | Analytical Letters |
| Volume | 44 |
| Issue | 17 |
| Page Numbers | 2834-2840 |
| Abstract | In this study, we reported that a bioluminescent assay for nitric oxide (NO) was developed utilizing the biological enzyme activity of soluble guanylate cyclase (sGC) and a bioluminescent assay for pyrophosphate involving the pyruvate phosphate dikinase (PPDK)-luciferin/Luciferase reaction. Pyrophosphate (PPi), which is released concomitantly when NO binds to soluble guanylate cyclase, was measured employing the PPDK/Luciferase reaction. NO binds to soluble guanylate cyclase specifically; thus, this assay is undisturbed by nitrate anhydride, NO(2)(-) or NO(3)(-). Consequently, the measurable range of NO obtained in the proposed method is 200-20,000 nM; the detection limit was 200 fmol/assay. NO released from nitrate medicine was measured utilizing this assay. As a result, NO released from isosorbide nitrate was detected. In conclusion, we suggest in this report that the technique for NO might be suitable as a quality check method for the pharmacodynamic action of nitrate medicines and in development of new medicines. |
| Doi | 10.1080/00032719.2011.565446 |
| Wosid | WOS:000298080200009 |
| Is Certified Translation | No |
| Dupe Override | No |
| Comments | Source: Web of Science WOS:000298080200009 |
| Is Public | Yes |
| Keyword | Bioluminescent assay; Guanylate cyclase; Luciferase; Nitric oxide |