Anti-inflammatory activity of brown alga Dictyota dichotoma in murine macrophage RAW 264.7 cells
Yoon, WJ; Ham, Y; Kim, K; Park, S; Lee, N; Hyun, CGu; Lee, W
| HERO ID | 1575718 |
|---|---|
| In Press | No |
| Year | 2009 |
| Title | Anti-inflammatory activity of brown alga Dictyota dichotoma in murine macrophage RAW 264.7 cells |
| Authors | Yoon, WJ; Ham, Y; Kim, K; Park, S; Lee, N; Hyun, CGu; Lee, W |
| Journal | Journal of Medicinal Plant Research |
| Volume | 3 |
| Issue | 1 |
| Page Numbers | 1-8 |
| Abstract | COX-2 (Cyclooxygenase-2) and iNOS ( inducible nitric oxide synthase) are involved in various pathophysiological processes such as inflammation. In a search for inhibitors of COX-2 and iNOS, we found that extracts of Dictyota dichotoma inhibit Nitric oxide ( NO) and PGE(2) generation in LPS-stimulated RAW 264.7 macrophage cells, as well as levels of iNOS and COX-2 mRNA and protein. D. dichotoma were extracted with 80% EtOH. The extract was then partitioned with hexane, CH2Cl2, EtOAc, BuOH, and water, successively. The results indicate that the CH2Cl2 fraction of D. dichotoma extract is an effective inhibitor of LPS-induced NO and PGE(2) production in RAW 264.7 cells. These inhibitory effects of the CH2Cl2 fraction of D. dichotoma were accompanied by decreases in the expression of iNOS and COX-2 proteins and iNOS and COX-2 mRNA in dose-dependent pattern. To test the inhibition effects of D. dichotoma fractions on other cytokines, we also performed RT-PCR on TNF-alpha, IL-1 beta, and IL-6 in LPS-stimulated RAW 264.7 macrophage cells. In these assays, the CH2Cl2 fraction of D. dichotoma also showed decreases in the expression of TNF-alpha, IL-1 beta, and IL-6 mRNA. Based on these results, we suggest that D. dichotoma extracts may be considered possible anti-inflammatory candidates for human health. |
| Wosid | WOS:000262157400001 |
| Is Certified Translation | No |
| Dupe Override | No |
| Comments | Source: Web of Science WOS:000262157400001Scopus URL: https://www.scopus.com/inward/record.uri?eid=2-s2.0-67349239168&partnerID=40&md5=c0cac3ff1a8ccdfdd0aa64a5f2ab8dbc |
| Is Public | Yes |
| Language Text | English |
| Keyword | COX-2; Cytokine; Dictyota dichotoma; inflammation; iNOS (inducible nitric oxide synthase); PGE(2) |