Bioavailability of the genotoxic components in coal tar contaminated soils in Fischer 344 rats
Bordelon, NR; Donnelly, KC; King, LC; Wolf, DC; Reeves, WR; George, SE
HERO ID
1576667
Reference Type
Journal Article
Year
2000
Language
English
PMID
| HERO ID | 1576667 |
|---|---|
| In Press | No |
| Year | 2000 |
| Title | Bioavailability of the genotoxic components in coal tar contaminated soils in Fischer 344 rats |
| Authors | Bordelon, NR; Donnelly, KC; King, LC; Wolf, DC; Reeves, WR; George, SE |
| Journal | Toxicological Sciences |
| Volume | 56 |
| Issue | 1 |
| Page Numbers | 37-48 |
| Abstract | The effect of chemical aging on the bioavailability and subsequent genotoxicity of coal tar (CT)-contaminated soils was evaluated in a 17-day feeding study using Fischer 344 male rats. Rats consumed a control diet or diets amended with soil, 0.35% CT, or soil freshly prepared or aged for 9 months with 0.35% CT. Mild treatment-related microscopic lesions in liver tissue and elevated enzyme levels in serum were detected in all CT treatment groups. The (32)P-postlabeling assay was employed to determine DNA adduct formation in treated animals. All CT treatment groups induced DNA adducts in both the liver and lung. Adduct levels were 3-fold higher in lung DNA compared to hepatic DNA. After correcting adduct levels for total ingested polycyclic aromatic hydrocarbons (PAHs), a significant decrease (p < 0.05) in adduct levels was observed in both CT/soil treatment groups compared to CT control in liver and lung DNA. Adduct profiles of (32)P-postlabeled hepatic and lung DNA displayed several nonpolar DNA adducts that comigrated with PAH-adducted calf thymus DNA standards as determined through both thin-layer chromatography (TLC) and high-pressure liquid chromatography (HPLC). These results suggest that soil, but not aging of contaminants in soil, decreases the bioavailability of genotoxic components in CT, as evidenced by DNA adduct analysis. |
| Doi | 10.1093/toxsci/56.1.37 |
| Pmid | 10869452 |
| Wosid | WOS:000087942900008 |
| Is Certified Translation | No |
| Dupe Override | No |
| Is Public | Yes |
| Language Text | English |