Reproductive tract malformations in rats following in utero and lactational exposure to di(n-butyl)-phthalate
Mylchreest, E; Foster, PM
HERO ID
1599672
Reference Type
Journal Article
Subtype
Abstract
Year
1997
Language
English
| HERO ID | 1599672 |
|---|---|
| Material Type | Abstract |
| In Press | No |
| Year | 1997 |
| Title | Reproductive tract malformations in rats following in utero and lactational exposure to di(n-butyl)-phthalate |
| Authors | Mylchreest, E; Foster, PM |
| Journal | Teratology |
| Volume | 55 |
| Issue | 1 |
| Page Numbers | 65 |
| Abstract | A reproductive assessment by continuous breeding study of di(n-butyl)-phthalate (DBP) was conducted by the US National Toxicology Program (NTP). When this plasticizing agent was administered continuously to rats of both sexes, fertility and reproductive tract effects were detected in adult F1 offspring, not in the F0 parents. The present study investigated the potential adverse effects on reproduction of in utero and lactational exposure of rats to DBP at dose levels similar to the NTP study, but with a shorter dosing period and production of a single litter. Pregnant CD rats (sperm-positive = gestation day (GD) 0; 10 dams/dose) were given DBP by gavage at 0, 250, 500 or 750 mg/kg body weight/day from GD 3 throughout pregnancy and lactation until the offspring were at postnatal day (PND) 20. Maternal body weights throughout the dosing period were not significantly affected by DBP treatment, and no clinical signs of toxicity were observed. DBP treatment had no apparent effect on parturition. Litter size was decreased in the 750 mg/kg/day group (9.0 +/- 0.7 vs 12.7 +/- 0.8 pups/litter in the control group). The number of implantation sites (on PND 21), proportion of pups born alive, sex ratio of live pups, and weight at birth were comparable in all groups. Adverse effects on the male reproductive system were induced in a dose-dependent manner. Anogenital distance on PND 2 was significantly decreased in males from dams treated with 500 and 750 mg DBP/kg/day. Undescended testes on PND 40 were observed in 0, 13, 29, and 25% of litters at 0, 250, 500, and 750 mg/kg/day, respectively. Small malformed prepuces and penises occured in 86 and 50% of litters at 500 and 750 mg/kg/day, respectively. The age at onset of vaginal opening and preputial separation was comparable in all groups, including gross morphologically normal males at 500 and 750 mg/kg/day. Decreased testicular size and poorly developed or absent epididymis were observed in all DBP groups. Lack of patent vagina and malformed or absent uteri and ovaries occured at 500 and 750 mg DBP/kg/day. Thus, exposure to high doses of DBP in utero and during the entire lactational period induced profound reproductive tract malformations in rats similar to those observed in the NTP study. |
| Wosid | BCI:BCI199799627137 |
| Is Certified Translation | No |
| Dupe Override | No |
| Conference Location | Palm Beach, FL |
| Conference Name | Joint Meeting of the Teratology Society Thirty-seventh Annual Meeting and Tenth International Conference of the Organization of Teratology Information Services |
| Conference Date | June 21-26, 1997 |
| Is Public | Yes |
| Language Text | English |
| Relationship(s) |
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