Monitoring "promiscuous" drug effects on single cells of multiple cell types
Kuang, Y; Walt, DR
| HERO ID | 1617082 |
|---|---|
| In Press | No |
| Year | 2005 |
| Title | Monitoring "promiscuous" drug effects on single cells of multiple cell types |
| Authors | Kuang, Y; Walt, DR |
| Journal | Analytical Biochemistry |
| Volume | 345 |
| Issue | 2 |
| Page Numbers | 320-325 |
| Abstract | Recent advances in genomics and molecular pathogenesis studies have determined that many diseases are caused by a multiplicity of factors. New drug regimens may consist of multiple biologically active agents designed to act synergistically on multiple biochemical targets. Live cell assays are becoming a standard for identifying new drug candidates with an emphasis on "homogeneous" living cell assays in which multiple cell lines are mixed and monitored simultaneously. In this study, we used a high-density single living cell array, based on an optical imaging fiber bundle microwell array, to simultaneously monitor "promiscuous" drug effects on single cells of multiple cell types. Such a capability allows for a more comprehensive understanding of how cells dynamically respond to combinatorial drug libraries or how different cellular pathways and regulation circuits respond cooperatively to drugs in individual cells. |
| Doi | 10.1016/j.ab.2005.06.028 |
| Pmid | 16099418 |
| Is Certified Translation | No |
| Dupe Override | No |
| Is Public | Yes |
| Language Text | English |