Oxidative stress and immune related gene expression following exposure to di-n-butyl phthalate and diethyl phthalate in zebrafish embryos
Xu, HJ; Shao, X; Zhang, Z; Zou, Y; Wu, X; Yang, L
HERO ID
1639200
Reference Type
Journal Article
Year
2013
Language
English
PMID
| HERO ID | 1639200 |
|---|---|
| In Press | No |
| Year | 2013 |
| Title | Oxidative stress and immune related gene expression following exposure to di-n-butyl phthalate and diethyl phthalate in zebrafish embryos |
| Authors | Xu, HJ; Shao, X; Zhang, Z; Zou, Y; Wu, X; Yang, L |
| Journal | Ecotoxicology and Environmental Safety |
| Volume | 93 |
| Page Numbers | 39-44 |
| Abstract | In the present study, we analyzed the oxidative stress related indices and immune related gene expression of zebrafish embryos after a short-term exposure to various concentrations of di-n-butyl phthalate (DBP), diethyl phthalate (DEP) and their mixture (DBP-DEP) from 4h post-fertilization (hpf) to 96hpf. Exposure to the chemicals was found to enhance the production of reactive oxygen species (ROS) and lipid peroxidation (LPO) in a concentration-dependent manner. Simultaneously, adaptive responses to DBP/DEP-induced oxidative stress were observed. The activity of antioxidant enzymes including superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPx) were all increased in a concentration-dependent manner. The transcription of innate immune related genes including interferon γ (IFNγ), interleukin-1β (IL1β), Myxovirus resistance (Mx), tumor necrosis factor α (TNFα), CC-chemokine, CXCL-clc, lysozyme (Lyz) and complement factor C3B (C3) were up-regulated upon DBP, DEP and their mixture exposure, suggesting the induction of immune response. In addition, co-exposure to DBP-DEP also induced antioxidant defense and immune response in zebrafish embryo. The results demonstrat that DBP/DEP exposure could induce the antioxidant and immune responses in zebrafish embryos. |
| Doi | 10.1016/j.ecoenv.2013.03.038 |
| Pmid | 23676468 |
| Wosid | WOS:000320080500006 |
| Url | https://search.proquest.com/docview/1357494103?accountid=171501 |
| Is Certified Translation | No |
| Dupe Override | No |
| Is Public | Yes |
| Language Text | English |
| Keyword | Oxidative stress; Immune response; Phthalate esters; Fish embryo |
| Is Peer Review | Yes |