[Effects of dibutyl phthalate and monobutyl phthalate on testosterone secretion and insulin-like factor 3 expression of Leydig tumor cells in mice]
Zhou, QH; Chen, X; Leng, L; Zhang, JS; Tang, NJ
| HERO ID | 1639204 |
|---|---|
| In Press | No |
| Year | 2013 |
| Title | [Effects of dibutyl phthalate and monobutyl phthalate on testosterone secretion and insulin-like factor 3 expression of Leydig tumor cells in mice] |
| Authors | Zhou, QH; Chen, X; Leng, L; Zhang, JS; Tang, NJ |
| Journal | Zhonghua Laodong Weisheng Zhiyebing Zazhi / Chinese Journal of Industrial Hygiene and Occupational Diseases |
| Volume | 31 |
| Issue | 2 |
| Page Numbers | 83-87 |
| Abstract | <strong>OBJECTIVE: </strong>To observe the effects of dibutyl phthalate (DBP) andmonobutyl phthalate (MBP) on the mRNA and protein expression of insulin-like factor 3 (INSL3) in the Leydig tumor cells (MA-10) of mice and the level of testosterone secreted from MA-10 cells.<br /><br /><strong>METHODS: </strong>The MA-10 cells of mice, used as a cellular model, were exposed to DBP and MBP. The content of testosterone in the supernatant medium was measured by enzyme-linked immunosorbent assay; the mRNA and protein expression levels of INSL3 in MA-10 cells were measured by quantitative PCR and Western Blot.<br /><br /><strong>RESULTS: </strong>Compared with the control group, MA-10 cells showed increased synthesis of testosterone when exposed to low concentrations of DBP and MBP (10(-9) ∼ 10(-6) mol/L) and inhibited synthesis of testosterone when exposed to high concentrations of DBP and MBP (10(-3) mol/L), and the typical two-way effects became more significant as the time went one and the concentrations increased (P < 0.05). Compared with the control group, MA-10 cells showed significantly lower mRNA and protein expression levels of INSL3 when exposed to 10(-6) and 10(-4) mol/L DBP (P < 0.05); MA-10 cells showed increased protein expression of INSL3 when exposed to 10(-7) mol/L MBP, and the mRNA and protein expression levels of INSL3 decreased as the concentration of MBP increased.<br /><br /><strong>CONCLUSION: </strong>DBP and MBP can inhibit the secretion of testosterone from MA-10 cells at high concentrations, but stimulate the secretion of testosterone at low concentrations. Both DBP and MBP have inhibitory effects on the mRNA and protein expression of INSL3 in MA-10 cells. |
| Pmid | 23433205 |
| Is Certified Translation | No |
| Dupe Override | No |
| Is Public | Yes |
| Language Text | Chinese |