A Japanese herbal medicine, Sho-saiko-to, prevents gut ischemia/reperfusion-induced hepatic microvascular dysfunction in rats
Horie, Y; Kajihara, M; Yamagishi, Y; Kimura, H; Tamai, H; Kato, S; Ishii, H
| HERO ID | 1656272 |
|---|---|
| In Press | No |
| Year | 2001 |
| Title | A Japanese herbal medicine, Sho-saiko-to, prevents gut ischemia/reperfusion-induced hepatic microvascular dysfunction in rats |
| Authors | Horie, Y; Kajihara, M; Yamagishi, Y; Kimura, H; Tamai, H; Kato, S; Ishii, H |
| Journal | Journal of Gastroenterology and Hepatology |
| Volume | 16 |
| Issue | 11 |
| Page Numbers | 1260-1266 |
| Abstract | <strong>BACKGROUND AND AIM: </strong>We have reported that gut ischemia/reperfusion (I/R) causes hepatic microvascular dysfunction. Nitric oxide (NO) has been found to be a modulator of the adhesive interactions between leukocytes, platelets, and endothelial cells. Sho-saiko-to (TJ-9), a Japanese herbal medicine, is reported to have protective effects against liver injury and to regulate NO production. The objective of this study was to determine whether TJ-9 affects hepatic microvascular dysfunction elicited by gut I/R, and to investigate the role of NO.<br /><br /><strong>METHODS: </strong>Male Wistar rats were exposed to 30 min of gut ischemia followed by 60 min of reperfusion. Intravital microscopy was used to monitor leukocyte recruitment and the number of non-perfused sinusoids (NPS). Plasma tumor necrosis factor (TNF)-alpha and alanine aminotransferase (ALT) activities were measured. In another set of experiments, TJ-9 (1 g/kg per day intragastrically) was administered to rats for 7 days. In some experiments, dexamethasone (ST) (2 mg/kg per day intravenously) was administered.<br /><br /><strong>RESULTS: </strong>In control rats, gut I/R elicited increases in the number of stationary leukocytes, NPS, and plasma TNF-alpha and ALT activities, and these changes were mitigated by the pretreatment with TJ-9. Pretreatment with an NO synthase inhibitor diminished the protective effects of TJ-9 on the increase in leukostasis in the pericentral region, NPS, and plasma TNF-alpha levels, but not its effect on the increase in leukostasis in the midzonal region, total number of stationary leukocytes, or plasma ALT activities. Pretreatment with TJ-9 increased plasma nitrite/nitrate levels. The responses caused by gut I/R were attenuated by the pretreatment with ST. Pretreatment with an NO synthase inhibitor did not affect the effect of ST.<br /><br /><strong>CONCLUSIONS: </strong>These results suggest that TJ-9 attenuates the gut I/R-induced hepatic microvascular dysfunction and inflammatory responses such as TNF-alpha production in the early phase via enhancement of NO production, and sequential hepatocellular damage via its anti-inflammatory effect like corticosteroid effect. |
| Pmid | 11903745 |
| Wosid | WOS:000179451000015 |
| Url | https://onlinelibrary.wiley.com/doi/10.1046/j.1440-1746.2001.02622.x |
| Is Certified Translation | No |
| Dupe Override | No |
| Is Public | Yes |
| Language Text | English |
| Keyword | corticosteroid; herbal medicine; intravital microscope; nitric oxide; tissue hypoxia |