Quantifying the effects of 1,1,1-trichloroethane and 1,1-dichloroethane on chlorinated ethene reductive dehalogenases
Chan, WW; Grostern, A; Löffler, FE; Edwards, EA
| HERO ID | 1738663 |
|---|---|
| In Press | No |
| Year | 2011 |
| Title | Quantifying the effects of 1,1,1-trichloroethane and 1,1-dichloroethane on chlorinated ethene reductive dehalogenases |
| Authors | Chan, WW; Grostern, A; Löffler, FE; Edwards, EA |
| Journal | Environmental Science & Technology |
| Volume | 45 |
| Issue | 22 |
| Page Numbers | 9693-9702 |
| Abstract | Mixtures of chlorinated ethenes and ethanes are often found at contaminated sites. In this study, we undertook a systematic investigation of the inhibitory effects of 1,1,1-trichloroethane (1,1,1-TCA) and 1,1-dichloroethane (1,1-DCA) on chlorinated ethene dechlorination in three distinct Dehalococcoides-containing consortia. To focus on inhibition acting directly on the reductive dehalogenases, dechlorination assays used cell-free extracts prepared from cultures actively dechlorinating trichloroethene (TCE) to ethene. The dechlorination assays were initiated with TCE, cis-1,2-dichloroethene (cDCE), or vinyl chloride (VC) as substrates and either 1,1,1-TCA or 1,1-DCA as potential inhibitors. 1,1,1-TCA inhibited VC dechlorination similarly in cell suspension and cell-free extract assays, implicating an effect on the VC reductases associated with the dechlorination of VC to nontoxic ethene. Concentrations of 1,1,1-TCA in the range of 30-270 μg/L reduced VC dechlorination rates by approximately 50% relative to conditions without 1,1,1-TCA. 1,1,1-TCA also inhibited reductive dehalogenases involved in TCE and cDCE dechlorination. In contrast, 1,1-DCA had no pronounced inhibitory effects on chlorinated ethene reductive dehalogenases, indicating that removal of 1,1,1-TCA via reductive dechlorination to 1,1-DCA is a viable strategy to relieve inhibition. |
| Doi | 10.1021/es201260n |
| Pmid | 21955221 |
| Wosid | WOS:000296756500031 |
| Url | https://search.proquest.com/docview/906377205?accountid=171501 |
| Is Certified Translation | No |
| Dupe Override | No |
| Comments | Source: Web of Science WOS:000296756500031 |
| Is Public | Yes |
| Language Text | English |
| Keyword | Environmental Studies; Enzymes; Chlorine; Water treatment; Nutrient removal; Ethylene |