Effect of S-adenosylmethionine on lipid peroxidation and liver fibrogenesis in carbon tetrachloride-induced cirrhosis
Gassó, M; Rubio, M; Varela, G; Cabré, M; Caballería, J; Alonso, E; Deulofem, R; Camps, J; Giménez, A; Pajares, M; Parés, A; Mato, JM; Rodés, J
HERO ID
194166
Reference Type
Journal Article
Year
1996
Language
English
PMID
| HERO ID | 194166 |
|---|---|
| In Press | No |
| Year | 1996 |
| Title | Effect of S-adenosylmethionine on lipid peroxidation and liver fibrogenesis in carbon tetrachloride-induced cirrhosis |
| Authors | Gassó, M; Rubio, M; Varela, G; Cabré, M; Caballería, J; Alonso, E; Deulofem, R; Camps, J; Giménez, A; Pajares, M; Parés, A; Mato, JM; Rodés, J |
| Journal | Journal of Hepatology |
| Volume | 25 |
| Issue | 2 |
| Page Numbers | 200-205 |
| Abstract | Background/Aim: The aim of this study was to investigate the effects of S-adenosylmethionine on liver peroxidation and liver fibrogenesis in carbon tetrachloride-induced cirrhosis. Methods: Cirrhosis was induced in three groups of six rats by repeated injections of carbon tetrachloride over 9 weeks. One group of animals was treated with carbon tetrachloride, and the other two received carbon tetrachloride plus S-adenosyl-methionine (10 mg/kg intramuscularly daily) from week 3 to week 9, and from week 6 to week 9 of the study, respectively. Two additional groups of six rats, a control group and a group treated only with S-adenosylmethionine, were also studied. Glutathione concentration, thiobarbituric acid-reactive substances, collagen content, prolyl hydroxylase activity, and procollagen type I mRNA expression were determined in liver samples. Results: All carbon tetrachloride-treated rats had cirrhosis at the end of the study. Cirrhosis was also present in five of the six carbon tetrachloride-treated rats received S-adenosylmethionine for 3 weeks, but in only one of the six rats that received S-adenosylmethionine for 6 weeks. Hepatic glutathione was significantly diminished in carbon tetrachloride-treated rats (2.7±0.3 μmol/g tissue) and returned to normal in rats received S-adenosylmethionine for 3 or 6 weeks (3.7±0.13 and 3.9±0.11 μmol/g tissue, respectively). The hepatic thiobarbituric acid-reactive substances were significantly lower in rats treated with carbon tetrachloride and S-adenosyl-methionine for 6 weeks (98±5 nmol/g) than in rats treated with carbon tetrachloride (134±12 nmol/g) and in those treated with carbon tetrachloride and S-adenosylmethionine for 3 weeks (127±13 nmol/g). There were no differences in either hepatic collagen and prolyl hydroxylase activity between rats that received only carbon tetrachloride and those treated with S-adenosylmethionine for 3 weeks. In contrast, carbon tetrachloride-treated rats received S-adenosylmethionine for 6 weeks had significantly lower collagen and prolyl hydroxylase activity than the other two groups. A much greater increase in procollagen type I mRNA was found in carbon tetrachloride-treated rats than in rats treated with carbon tetrachloride and S-adenosylmethionine for 6 weeks. Furthermore, there was a significant correlation between the hepatic thiobarbituric acid-reactive substances and prolyl hydroxylase activity and hepatic collagen. Conclusions: We conclude that the early administration of S-adenosylmethionine in a model of carbon tetrachloride-induced liver injury restores glutathione levels and reduces lipid peroxidation, resulting in less advanced liver fibrosis. |
| Doi | 10.1016/S0168-8278(96)80074-2 |
| Pmid | 8878782 |
| Wosid | WOS:A1996VE41600012 |
| Is Certified Translation | No |
| Dupe Override | No |
| Is Public | Yes |
| Language Text | English |
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