Decrease in protein kinase and phosphatase activities in the liver nuclei of rats exposed to carbon tetrachloride
Omura, M; Katsumata, T; Misawa, H; Yamaguchi, M
HERO ID
194716
Reference Type
Journal Article
Year
1999
Language
English
PMID
| HERO ID | 194716 |
|---|---|
| In Press | No |
| Year | 1999 |
| Title | Decrease in protein kinase and phosphatase activities in the liver nuclei of rats exposed to carbon tetrachloride |
| Authors | Omura, M; Katsumata, T; Misawa, H; Yamaguchi, M |
| Journal | Toxicology and Applied Pharmacology |
| Volume | 160 |
| Issue | 2 |
| Page Numbers | 192-197 |
| Abstract | The alteration in protein kinase and phosphatase activities in the liver nuclei of rats administered carbon tetrachloride (CCl(4)) was investigated. Rats received a single oral administration of CCl(4) (1 ml/100 g body wt of 5, 10, and 25% CCl(4) in corn oil), and 5, 24, and 48 h later they were euthanized by bleeding. The administration of CCl(4) (10 and 25%) caused a significant decrease in protein kinase activity in the liver nuclei. The enzyme activity in the liver nuclei from normal and CCl(4)-administered rats was significantly increased by the addition of Ca(2+) (0.5 mM) and calmodulin (10 microg/ml) in the reaction mixture, suggesting that Ca(2+)/calmodulin-dependent protein kinase activation is not suppressed by CCl(4) treatment. Liver nuclear phosphatase activity toward phosphotyrosine, but not phosphoserine and phosphothreonine, was markedly decreased by CCl(4) (5, 10, and 25%) administration. This decrease was seen 5 h after CCl(4) administration. The presence of vanadate (10(-4) M) in the reaction mixture caused a significant decrease in phosphotyrosine phosphatase activity in the liver nuclei from normal and CCl(4)-administered rats, whereas the enzyme activity was not decreased by okadaic acid (10(-5) M) or sodium fluoride (10(-3) M). The effect of anti-regucalcin antibody (100 ng/ml) in increasing phosphotyrosine phosphatase activity was seen in the liver nuclei of CCl(4)-administered rats, suggesting that regucalcin-sensitive phosphatase activity is decreased by CCl(4) administration. The present study demonstrates that CCl(4) administration induces a decrease in protein kinase and tyrosine phosphatase activities, which are involved in signaling factors in the liver nuclei of rats. Copyright 1999 Academic Press. |
| Doi | 10.1006/taap.1999.8760 |
| Pmid | 10527918 |
| Wosid | WOS:000083405200010 |
| Is Certified Translation | No |
| Dupe Override | No |
| Comments | Source: Web of Science WOS:000083405200010 |
| Is Public | Yes |
| Language Text | English |
| Keyword | protein kinase; protein phosphatase; regucalcin; liver nuclei; carbon tetrachloride |
| Is Qa | No |