Carbon tetrachloride suppresses T-cell-dependent immune responses by induction of transforming growth factor-beta 1
Delaney, B; Strom, SC; Collins, S; Kaminski, NE
| HERO ID | 194748 |
|---|---|
| In Press | No |
| Year | 1994 |
| Title | Carbon tetrachloride suppresses T-cell-dependent immune responses by induction of transforming growth factor-beta 1 |
| Authors | Delaney, B; Strom, SC; Collins, S; Kaminski, NE |
| Journal | Toxicology and Applied Pharmacology |
| Volume | 126 |
| Issue | 1 |
| Page Numbers | 98-107 |
| Abstract | In vivo exposure of B6C3F1 mice to the hepatotoxic chlorinated hydrocarbon, CCl4, suppresses T-cell-dependent humoral immune responses to sRBC. In the present studies, separation-crossover-reconstitution experiments with spleen cell subpopulations isolated from vehicle and CCl4-treated mice (500 mg/kg/day for 7 days) indicate that T-cells are the primary immunologic cell-type altered following CCl4 exposure. Despite suppression of T-cell activity, Con A-activated spleen cell supernatants from CCl4-treated mice produced greater amounts of biologically active IL-2 than untreated spleen cells. Furthermore, Con A-induced upregulation of the p55 subunit of the IL-2 receptor was not altered in spleen cells from CCl4-treated mice. The mediator of immune suppression is a serum-borne factor induced 48 hr following a single exposure to CCl4. Sera isolated from mice treated with CCl4 for 7 days (500 mg/kg/day) or 48 hr following a single exposure (1000 mg/kg) were found to possess high concentrations of TGF-beta 1. Direct addition studies demonstrated that T-cell-dependent AFC responses are more sensitive to suppression by TGF-beta 1 than are T-cell-independent responses. Finally, incubation of sera from CCl4-treated mice with TGF-beta 1-neutralizing mAb reversed the immune suppression associated with this serum. These results demonstrate that CCl4-induced immune suppression is at least partially mediated by induction of TGF-beta 1. |
| Doi | 10.1006/taap.1994.1095 |
| Pmid | 8184439 |
| Wosid | WOS:A1994NL85400013 |
| Is Certified Translation | No |
| Dupe Override | No |
| Is Public | Yes |
| Language Text | English |
| Is Qa | No |