Covalent binding of carbon tetrachloride metabolites to liver nuclear DNA, proteins, and lipids

Diaz Gomez, MI; Castro, JA

HERO ID

194790

Reference Type

Journal Article

Year

1980

Language

English

PMID

7466819

HERO ID 194790
In Press No
Year 1980
Title Covalent binding of carbon tetrachloride metabolites to liver nuclear DNA, proteins, and lipids
Authors Diaz Gomez, MI; Castro, JA
Journal Toxicology and Applied Pharmacology
Volume 56
Issue 2
Page Numbers 199-206
Abstract Abstract: super(1) super(4)C from super(1) super(4)CCl sub(4) irreversibly bind in vivo) to liver DNA from strain A/J mice and Sprague-Dawley rats. Binding of super(1) super(4)CCl sub(4) to DNA was also observed in vitro) in incubation mixtures containing microsomes and a NADPH-generating system as well as in tissue slices. Chemically induced multiplied by CCl sub(3) (CCl sub(4) + benzoyl peroxide system) intensively binds to DNA. Liver nuclear proteins also irreversibly bind CCl sub(4) metabolites. Nuclear protein fractionation studies revealed that deoxyribonucleoproteins, acidic proteins, histones, and residual proteins are the favorite targets of metabolite interaction. Nuclear sap proteins are less intensively labeled. Nuclear lipids were markedly labeled by CCl sub(4) reactive metabolites. Most of the label is in the phospholipid fraction and diphosphatidylglycerol is most intensively labeled; phosphatidylethanolamine, phosphatidylcholine, lysophosphatidylcholine, and sphingomyeline are also labeled by metabolites. ds: carbon tetrachloride evised - 2006-11-01 e field[29]: 1,4-Dioxane
Pmid 7466819
Is Certified Translation No
Dupe Override No
Is Public Yes
Language Text English