Carbon tetrachloride-induced changes in adrenal microsomal mixed-function oxidases and lipid peroxidation
Colby, HD; Brogan, WC; Miles, PR
HERO ID
195006
Reference Type
Journal Article
Year
1981
Language
English
PMID
| HERO ID | 195006 |
|---|---|
| In Press | No |
| Year | 1981 |
| Title | Carbon tetrachloride-induced changes in adrenal microsomal mixed-function oxidases and lipid peroxidation |
| Authors | Colby, HD; Brogan, WC; Miles, PR |
| Journal | Toxicology and Applied Pharmacology |
| Volume | 60 |
| Issue | 3 |
| Page Numbers | 492-499 |
| Abstract | Studies were carried out to compare the effects of CCl4 in vivo and in vitro on adrenal and hepatic microsomal metabolism in guinea pigs. CCl4 administration in vivo decreased adrenal and hepatic microsomal cytochrome P-450 concentrations and lowered benzphetamine (BZ) demethylase and benzo(a)pyrene (BP) hydroxylase activities in both tissues. NADPH-cytochrome c reductase activity was decreased in hepatic but not in adrenal microsomes. Addition of CCl4 to adrenal or hepatic microsomes in vitro produced a type 1 difference spectrum suggesting binding of CCl4 to cytochrome(s) P-450; its magnitude was greater in adrenal than in liver. Incubation of adrenal or hepatic microsomes in vitro with CCl4 alone had little or no effect on mixed-function oxidase activity or on lipid peroxidation. When microsomes were incubated with CCl4 + NADPH, the rates of BZ and BP metabolism decreased, cytochrome P-450 concentrations decreased and lipid peroxidation was increased. The effects of CCl4 + NADPH on enzyme activities were greater in adrenal than in hepatic microsomes. Addition of 1.0 mM EDTA or 0.1 mM MnCl2 to the incubation medium blocked the effects of CCl4 + NADPH on lipid peroxidation in adrenal and liver but had no effect on the decreases in mixed-function oxidase activities. The adrenal cortex in the guinea pig was an active site of CCl4 metabolism. CCl4 metabolism resulted in a loss of microsomal enzyme activities in the adrenal and liver. Lipid peroxidation was not obligatory for CCl4-mediated destruction of microsomal enzymes. |
| Doi | 10.1016/0041-008X(81)90336-7 |
| Pmid | 7292488 |
| Wosid | WOS:A1981MK92500014 |
| Is Certified Translation | No |
| Dupe Override | No |
| Comments | Scopus URL: https://www.scopus.com/inward/record.uri?eid=2-s2.0-0019843835&doi=10.1016%2f0041-008X%2881%2990336-7&partnerID=40&md5=5757f8f439a0fbe164c5c3e57897d9ea |
| Is Public | Yes |
| Language Text | English |
| Relationship(s) |
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