Regulation of salicylic acid synthesis in ozone-exposed tobacco and Arabidopsis
Ogawa, D; Nakajima, N; Sano, T; Tamaoki, M; Aono, M; Kubo, A; Kamada, H; Saji, H
| HERO ID | 199209 |
|---|---|
| In Press | No |
| Year | 2005 |
| Title | Regulation of salicylic acid synthesis in ozone-exposed tobacco and Arabidopsis |
| Authors | Ogawa, D; Nakajima, N; Sano, T; Tamaoki, M; Aono, M; Kubo, A; Kamada, H; Saji, H |
| Journal | Phyton-Annales Rei Botanicae |
| Volume | 45 |
| Issue | 4 |
| Page Numbers | 169-175 |
| Abstract | Ozone, a major photochemical oxidant, induces leaf injury. Salicylic acid (SA) is a kind of plant hormone and an important regulator of plant resistance to pathogens. In ozone-exposed plants, SA participates in both the formation of leaf injury and the defense response. In pathogen-infected plants, SA is synthesized via two pathways involving phenylalanine or isochorismate. Biosynthesis of SA in ozone-fumigated plants had not been well defined, so we examined it in tobacco and Arabidopsis. Salicylic acid accumulated in tobacco exposed to 0.2 ppm ozone for 6 h. At the same time, phenylalanine ammonia-lyase (PAL) activity, its mRNA level, and the level of chorismate mutase (CM) transcripts increased remarkably, whereas isochorismate synthase (ICS) activity did not increase. These results may suggest that ozone-exposed tobacco synthesized SA via the phenylalanine pathway. Salicylic acid levels also increased in ozone-exposed Arabidopsis, but not in sid2 (salicylic acid induction-deficient 2) mutants, in which ICSI is defective. Furthermore, ICS activity and the mRNA level of ICSI increased dramatically in wild-type Arabidopsis after the start of ozone exposure. These results suggest that ozone-exposed Arabidopsis synthesizes SA from isochorismate. Therefore, our results imply that the main pathway of ozone-induced SA biosynthesis differs between tobacco and Arabidopsis. |
| Wosid | WOS:000241970700024 |
| Is Certified Translation | No |
| Dupe Override | No |
| Comments | N1-Regulation of salicylic acid synthesis in ozone-exposed tobacco and ArabidopsisN1-ISI:000241970700024N1-Ogawa, D. Nakajima, N. Sano, T. Tamaoki, M. Aono, M. Kubo, A. Kamada, H. Saji, H. Sp. Iss. SIAD-Univ Tsukuba, Grad Sch Life & Environm Sci, Div Funct Biosci, Tsukuba, Ibaraki 3058572, Japan. Natl Inst Environm Studies, Tsukuba, Ibaraki 3058506, Japan. Ogawa, D, Univ Tsukuba, Grad Sch Life & Environm Sci, Div Funct Biosci, Tsukuba, Ibaraki 3058572, Japan. ogawa.daisuke@nies.go.jpID - 1212 |
| Is Public | Yes |
| Keyword | phenylalanine ammonia-lyase; isochorismate synthase; chorismate mutase; PHENYLALANINE AMMONIA-LYASE; HYPERSENSITIVE CELL-DEATH; ISOCHORISMATE; SYNTHASE; PLANT DEFENSE; MOSAIC-VIRUS; BIOSYNTHESIS; ENZYMES; RESISTANCE; EXPRESSION; PATHWAY |
| Is Qa | No |