ABUNDANCE AND DIVERSITY OF AMMONIA-OXIDIZING BACTERIA IN RELATION TO AMMONIUM IN A CHINESE SHALLOW EUTROPHIC URBAN LAKE
Qiu, S; Chen, G; Zhou, Y
HERO ID
2020471
Reference Type
Journal Article
Year
2010
Language
English
PMID
| HERO ID | 2020471 |
|---|---|
| In Press | No |
| Year | 2010 |
| Title | ABUNDANCE AND DIVERSITY OF AMMONIA-OXIDIZING BACTERIA IN RELATION TO AMMONIUM IN A CHINESE SHALLOW EUTROPHIC URBAN LAKE |
| Authors | Qiu, S; Chen, G; Zhou, Y |
| Journal | Brazilian Journal of Microbiology |
| Volume | 41 |
| Issue | 1 |
| Page Numbers | 218-226 |
| Abstract | The measures of most-probable-number and restriction fragment length polymorphism analysis were used to analyze the abundance and diversity of ammonia-oxidizing bacteria in sediment of a Chinese shallow eutrophic urban lake (Lake Yuehu). Among the 5 sampling sites, ammonia concentration in interstitial water was positively proportional not only to the content of organic matter, but also to ammonia-oxidizing bacteria numbers (at a magnitude of 10(5) cells g(-1) dry weight) in sediment significantly. Furthermore, the diversity of ammonia-oxidizing bacteria were determined by means of PCR primers targeting the amoA gene with five gene libraries created and restriction pattern analysis. The 13 restriction patterns were recorded with 4 ones being common among all sampling sites. The 8 restriction patterns including 4 unique ones were found at the site with the highest NH4 (+) concentrations in interstitial water, while, there were only common patterns without unique ones at the site with the lowest NH4 (+) concentrations in interstitial water. Phylogenetic analysis showed that the amoA fragments retrieved belong to Nitrosomonas oligotropha & ureae lineage, N. europaea lineage, N. communis lineage and Nitrosospira lineage, most of which were affiliated with the genus Nitrosomonas. The N. oligotropha & ureae-like bacteria were the dominant species. Thus, the abundance and diversity of sediment AOB is closely linked to ammonium status in eutrophic lakes. |
| Doi | 10.1590/S1517-838220100001000031 |
| Pmid | 24031484 |
| Wosid | WOS:000293874200031 |
| Is Certified Translation | No |
| Dupe Override | No |
| Is Public | Yes |
| Language Text | English |
| Keyword | Ammonia-oxidizing bacteria; Abundance; amoA; Diversity |