Differential acid-base regulation in various gills of the green crab Carcinus maenas: Effects of elevated environmental pCO(2)
Fehsenfeld, S; Weihrauch, D
| HERO ID | 2041367 |
|---|---|
| In Press | No |
| Year | 2013 |
| Title | Differential acid-base regulation in various gills of the green crab Carcinus maenas: Effects of elevated environmental pCO(2) |
| Authors | Fehsenfeld, S; Weihrauch, D |
| Journal | Comparative Biochemistry and Physiology - Part A: Molecular and Integrative Physiology |
| Volume | 164 |
| Issue | 1 |
| Page Numbers | 54-65 |
| Abstract | Euryhaline decapod crustaceans possess an efficient regulation apparatus located in the gill epithelia, providing a high adaptation potential to varying environmental abiotic conditions. Even though many studies focussed on the osmoregulatory capacity of the gills, acid-base regulatory mechanisms have obtained much less attention. In the present study, underlying principles and effects of elevated pCO(2) on acid-base regulatory patterns were investigated in the green crab Carcinus maenas acclimated to diluted seawater. In gill perfusion experiments, all investigated gills 4-9 were observed to up-regulate the pH of the hemolymph by 0.1-0.2 units. Anterior gills, especially gill 4, were identified to be most efficient in the equivalent proton excretion rate. Ammonia excretion rates mirrored this pattern among gills, indicating a linkage between both processes. In specimen exposed to elevated pCO(2) levels for at least 7 days, mimicking a future ocean scenario as predicted until the year 2300, hemolymph K(+) and ammonia concentrations were significantly elevated, and an increased ammonia excretion rate was observed. A detailed quantitative gene expression analysis revealed that upon elevated pCO(2) exposure, mRNA levels of transcripts hypothesized to be involved in ammonia and acid-base regulation (Rhesus-like protein, membrane-bound carbonic anhydrase, Na(+)/K(+)-ATPase) were affected predominantly in the non-osmoregulating anterior gills. |
| Doi | 10.1016/j.cbpa.2012.09.016 |
| Pmid | 23022520 |
| Wosid | WOS:000312421400008 |
| Is Certified Translation | No |
| Dupe Override | No |
| Is Public | Yes |
| Language Text | English |
| Keyword | PH; Gill perfusion; Quantitative real-time PCR; Ammonia; Rhesus-like protein |