Comparative analysis of PAH : DNA adducts formed in lung of mice exposed to neat coal tar and soils contaminated with coal tar
Koganti, A; Singh, R; Ma, BL; Weyand, EH
| HERO ID | 2123183 |
|---|---|
| In Press | No |
| Year | 2001 |
| Title | Comparative analysis of PAH : DNA adducts formed in lung of mice exposed to neat coal tar and soils contaminated with coal tar |
| Authors | Koganti, A; Singh, R; Ma, BL; Weyand, EH |
| Journal | Environmental Science & Technology |
| Volume | 35 |
| Issue | 13 |
| Page Numbers | 2704-2709 |
| Abstract | 7H-Benzo[c]fluorene (benzo[c]fluorene) is a major DNA adduct forming component of coal tar in lung of mice. The present study evaluated the types of PAH:DNA adducts formed from different neat coal tar samples and soils contaminated with coal tar. Mice were fed diets containing coal tar either neat or as a contaminant in an environmental soil sample for 14 days, and the types of chemical:DNA adducts formed in lung were evaluated using P-32-postlabeling and HPLC analysis. Three major DNA adducts derived respectively from benzo[b]fluoranthene (B[b]F), benzo[a]pyrene (B[a]P), and benzo[c]fluorene were detected in three of the four neat coal tar samples evaluated. In contrast, only a single major DNA adduct derived from benzo[c]fluorene was observed with the remaining tar sample. Ingestion of coal tar contaminated soil resulted in DNA adducts primarily derived from benzo[c]fluorene and B[b]F; a B[a]P derived DNA adduct was not detected. The DNA adducts derived from benzo[c]fluorene and B[b]F but not B[a]P were also observed with animals fed methylene chloride extracts of three of these soils but not the one designated A1000H soil. However, the extract of A1000H resulted in a B[a]P: DNA adduct being detected along with adducts formed from B[b]F and benzo[c]fluorene. The selective formation of the benzo[c]fluorene:DNA adduct with coal tar contaminated soils indicates that the in vivo systemic bioavailability and/ or metabolism of benzo[c]fluorene is relatively high when compared to other DNA adducting hydrocarbons within coal tar. Benzo[c]fluorene may play a critical role in the potential of contaminated soil to induce a toxicological response in animals. |
| Doi | 10.1021/es001532i |
| Pmid | 11452595 |
| Wosid | WOS:000169600100029 |
| Is Certified Translation | No |
| Dupe Override | No |
| Comments | Scopus URL: https://www.scopus.com/inward/record.uri?eid=2-s2.0-0035387495&doi=10.1021%2fes001532i&partnerID=40&md5=1195600bdcad674b80192b8a4785d0f8 |
| Is Public | Yes |
| Language Text | English |