Tissue binding of [9-14C] 2-acetylaminofluorene in mice during oral administration

Jackson, CD; Weis, C; Shellenberger, TE

HERO ID

2147649

Reference Type

Journal Article

Subtype

Abstract

Year

1974

Language

English

HERO ID 2147649
Material Type Abstract
In Press No
Year 1974
Title Tissue binding of [9-14C] 2-acetylaminofluorene in mice during oral administration
Authors Jackson, CD; Weis, C; Shellenberger, TE
Journal Toxicology and Applied Pharmacology
Volume 29
Issue 1
Page Numbers 107
Abstract This study was made to determine the extent of tissue binding of 2-acetylaminofluorene (AAF) during oral administration of the carcinogen at different dose levels. Weanling, female BALB/cJ and C57BL/6J mice were maintained on diets containing 5 or 500 ppm [9-14C] AAF for 8 weeks. The amount of 14C bound to liver and bladder tissue, as well as blood concentrations, were determined at various intervals. Results obtained with the two dose levels were essentially parallel with amounts of 14C being approximately 100-fold higher in mice on the 500 ppm diet. Blood values of 14C in both strains of mice increased for 2 weeks and then remained constant with the concentrations in BALB/cJ mice being twice that of C57BL/6J mice on the same diet. Amounts of bound 14C in liver tissue also reached a plateau at 2 weeks and were approximately 40 % higher in C57BL/6J mice. In contrast, the amount of 14C bound to bladder tissue continued to increase over the entire 8-week period. In a second experiment, weanling, female BALB/cJ and C57BL/6J mice were maintained on diets containing AAF at various levels over the range of 0.5-500 ppm and sacrificed at the end of 2 weeks. Blood and tissue concentrations were determined as above. The results, plotted as log 14C bound vs log concentration of AAF in the diet, indicate that the amount of AAF bound to both liver and bladder tissue is linear over the dose range studied.
Wosid WOS:A1974T653800088
Is Certified Translation No
Dupe Override No
Conference Location Washington, D.C.
Conference Name Thirteenth Annual Meeting of the Society of Toxicology
Conference Date March 10–14, 1974
Is Public Yes
Language Text English
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