Persistence of PCB homologs and isomers in domestic hen and rat

Baker, FD; Bush, B; TUMASONI.C

HERO ID

2152979

Reference Type

Journal Article

Subtype

Abstract

Year

1974

Language

English

HERO ID 2152979
Material Type Abstract
In Press No
Year 1974
Title Persistence of PCB homologs and isomers in domestic hen and rat
Authors Baker, FD; Bush, B; TUMASONI.C
Journal Toxicology and Applied Pharmacology
Volume 29
Issue 1
Page Numbers 104-105
Abstract This study was undertaken to (1) separate, identify, and quantitate the 18 major component of Aroclor 1254 in adult hens and pregnant rats, in the embryos and fetuses, and in the progeny of both species during and after exposure; (2) compare the metabolic patterns of Aroclor 1254 for both species; (3) correlate toxicity and abnormalities with specific isomers; and (4) examine the possible relationship between induced mixed-function oxidase activity and hydroxylation of isomers. Aroclor 1254, emulsified in drinking water, was administered at a level of 6 mg/kg body weight (50 ppm) to hens and to rats over periods of 6 and 9 weeks respectively. After the intake was terminated, the populations were observed for 20 and 13 weeks respectively as the level of PCB declined. Egg yolks, tissues of viable 18-day-old embryos, and surviving 5-day-old chicks were analyzed by thin-layer chromatography and by gas chromatography during the course of the experiment. Data suggest that 4-4’chlorination is more important than degree of chlorination in determining persistence of chlorinated biphenyl in the hen, chick embryo and hatched chick. Results with both hens and rats indicate that fertility was not affected at the level of PCB employed and that the degree, and termination, of embryonic and/or fetal development was related directly to length of exposure. Mixed-function oxidase activity (MFO) on liver microsomal preparations from rat mothers, fetuses and pups was measured by determination of liver microsomal cytochrome P-450 and aniline hydroxylase levels. Preliminary results indicate sex-related MFO response.
Wosid WOS:A1974T653800081
Is Certified Translation No
Dupe Override No
Conference Location Washington, D.C.
Conference Name Thirteenth Annual Meeting of the Society of Toxicology
Conference Date March 10–14, 1974
Is Public Yes
Language Text English
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