Metabolism and excretion of 2,4,5,2',5'-pentachlorobiphenyl (PCB) in male rat
Chen, PR; Matthews, HB
HERO ID
2157179
Reference Type
Journal Article
Subtype
Abstract
Year
1974
Language
English
| HERO ID | 2157179 |
|---|---|
| Material Type | Abstract |
| In Press | No |
| Year | 1974 |
| Title | Metabolism and excretion of 2,4,5,2',5'-pentachlorobiphenyl (PCB) in male rat |
| Authors | Chen, PR; Matthews, HB |
| Journal | Toxicology and Applied Pharmacology |
| Volume | 29 |
| Issue | 1 |
| Page Numbers | 88 |
| Abstract | The metabolism and excretion of [14C]PCB in male rats was followed as part of a pharma cokinetic study. After iv doses of 0.6 or 6.0 mg/kg, excretion of PCB occurred primarily in the feces of rats with about 50% excreted during the first 3 days after dosing. Another 12% was excreted in feces during the next 4 days. About 5% of the dose of PCB was excreted in the urine during the first 24 hr with only an additional 1% excreted thereafter. Analysis of radio activity excreted in the feces revealed the presence of 2 metabolites as well as significant amounts of parent PCB. Both metabolites were isolated and analyzed by chemical ionization mass spectroscopy (MS). The data obtained from MS indicated the major metabolite was a mono hydroxylated-PCB and the minor metabolite was a dihydroxy analog. NMR studies are in progress to determine the positions of the hydroxyl groups. Analysis of urinary radioactivity revealed the presence of only 1 metabolite, a glucuronide conjugate. That metabolism is closely coupled to excretion is suggested by the fact that no metabolites of PCB were found in liver and other tissues. The size of the dose apparently had no effect on the rate of excretion. During the first 24 hr, the rate of excretion of PCB and metabolites in the feces was markedly increased by animal pretreatment with an inducer, phenobarbital, and decreased by an inhibitor, SKF-525A, of the hepatic microsomal mixed-function oxidases. In conclusion, PCB is excreted primarily via the biliary route in rats. Excretion occurs both in the form of hydroxylated metabolites and as unchanged PCB, and the excretion rate is sensitive to the effects of an inducer or inhibitor of the hepatic microsomal hydroxylating enzymes. |
| Wosid | WOS:A1974T653800040 |
| Is Certified Translation | No |
| Dupe Override | No |
| Conference Location | Washington, D.C. |
| Conference Name | Thirteenth Annual Meeting of the Society of Toxicology |
| Conference Date | March 10–14, 1974 |
| Is Public | Yes |
| Language Text | English |
| Relationship(s) |
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