Effects of mirex and chloroquine on PCB-induced hepatic porphyria in the rat
Fulfs, JC; Abraham, R
HERO ID
2200154
Reference Type
Journal Article
Subtype
Abstract
Year
1976
Language
English
| HERO ID | 2200154 |
|---|---|
| Material Type | Abstract |
| In Press | No |
| Year | 1976 |
| Title | Effects of mirex and chloroquine on PCB-induced hepatic porphyria in the rat |
| Authors | Fulfs, JC; Abraham, R |
| Journal | Toxicology and Applied Pharmacology |
| Volume | 37 |
| Issue | 1 |
| Page Numbers | 119-120 |
| Abstract | As a continuation of previous work, PCB (Aroclor 1221) and mirex were given to rats by oral gavage for up to 17 months: Group I, PCB (25 mg/kg); Group II, mirex (1.5 mg/kg); Group III, PCB (25 mg/kg) and mirex (1.5 mg/kg); and Group IV, corn oil. Hepatic centrilobular hypertrophy was initially observed at 3 months in all experimental groups but was most marked in Group III. This condition was enhanced at 6 and 17 months and in Group III hypertrophy of hepatocytes extended into the midzonal regions with occasional focal necrosis. Hepatocytes in the centrilobular area contained granules in Groups I and III, which were first noted at 6 months; their number increased with time. Lysosomal acid phosphatase activity was present at similar sites as the granules. These granules were identified as porphyrins as determined histochemically and biochemically. Ultrastructurally, the porphyrins appeared as dense granular inclusions within single membrane-bound vacuoles which contained acid phosphatase activity (lysosomes) and were localized near bile canaliculi. Group II livers had no such granules. At 17 months, an increase in porphyrin granules was observed in Group I and III. In Group III, however, these granules extended throughout the liver lobule. While mirex appeared to have enhanced for formation and retention of porphyrins in lysosomes in Group III, chloroquine had the exact opposite effect. Twenty-four hours following chloroquine treatment, there was a complete absence of ''porphyrin-laden lysosomes,'' suggesting a rapid labilization and exocytosis of these structures. Proliferation of smooth endoplasmic reticulum was noted in all experimental groups that was accompanied by a loss of glucose-6-phosphatase activity. (Author abstract by permission, abstract no. 67) |
| Wosid | WOS:A1976BZ49700076 |
| Is Certified Translation | No |
| Dupe Override | No |
| Comments | : |
| Is Public | Yes |
| Language Text | English |
| Relationship(s) |