KRAS2 as a genetic marker for lung tumor susceptibility in inbred mice
Ryan, J; Barker, PE; Nesbitt, MN; Ruddle, FH
| HERO ID | 2346989 |
|---|---|
| In Press | No |
| Year | 1987 |
| Title | KRAS2 as a genetic marker for lung tumor susceptibility in inbred mice |
| Authors | Ryan, J; Barker, PE; Nesbitt, MN; Ruddle, FH |
| Journal | Journal of the National Cancer Institute |
| Volume | 79 |
| Issue | 6 |
| Page Numbers | 1351-1357 |
| Abstract | An Eco-RI restriction fragment length polymorphism occurring in a DNA fragment containing the first exon of the murine KRAS2 gene was shown to correlate with the inherited susceptibility of inbred strains of mice to urethan (CAS: 51-79-6)-induced pulmonary adenomas. Eco-RI digestion of murine DNA yielded four KRAS2-specific fragments. Polymorphic variation occurred in the smallest molecular-weight fragment with alleles of either 0.70 or 0.55 kb in size. Genotyping of 14 inbred strains of mice revealed a correlation between KRAS2 Eco-RI polymorphic variation and the differential susceptibility among inbred strains to development of pulmonary adenomas. Strains with a high incidence of pulmonary adenomas, either spontaneously occurring or in response to carcinogen induction, had the 0.55-kb KRAS2 allele whereas adenoma-resistant strains had the 0.70-kb allele. Analysis of a series of recombinant inbred strains (AXB, BXA) that developed from reciprocal crosses between a highly susceptible strain (A/J) and a highly resistant strain (C57BL/6J) revealed a statistically significant threefold difference in lung tumor susceptibility on the basis of KRAS2 genotype. Further analysis of individual F2 mice of a C57BL/6 female X A/J male cross also demonstrated a threefold difference in tumor susceptibility on the basis of KRAS2 allelic variation. |
| Pmid | 2891865 |
| Is Certified Translation | No |
| Dupe Override | No |
| Is Public | Yes |
| Language Text | English |