Munitions Cytotoxicities In Vitro
Mitchell, W; Dasko-Vincent, L; Wellington, D
| HERO ID | 2799208 |
|---|---|
| Year | 1995 |
| Title | Munitions Cytotoxicities In Vitro |
| Authors | Mitchell, W; Dasko-Vincent, L; Wellington, D |
| Publisher Text | Edgewood Research Development and Engineering Center Aberdeen Proving Ground MD |
| Page Numbers | 7 |
| Abstract | Rapid, inexpensive, and reliable methods are needed for the toxicological screening of chemical substances with the potential to affect health and the environment. One such method, the neutral red (NR) cytotoxicity assay, is based on incorporation of the supravital dye neutral red into lysosomes of viable cells. The NR uptake assay can be used to detect cytotoxic or cytostatic effects of chemical substances capable of damaging cells, has been adapted to microtiter tissue culture systems, and can be analyzed by means of automated spectrophotometric microplate readers (1,2). Neutral red cytotoxicity assays in continuous rat hepatoma H4IIE cells have been applied to a variety of environ-mentally important munitions and related compounds. The H4IIE cells maintain inducible oxidative microsomal enzymes and were chosen because of their application to detecting other xenobiotics in environmental and biological specimens (6). |
| Report Number | ADA294804 |
| Url | https://www.dtic.mil/DTICOnline/downloadPdf.search?collectionId=tr&docId=ADA294804 |
| Is Certified Translation | No |
| Dupe Override | No |
| Comments | Journal: ISSN: |
| Is Public | Yes |
| Keyword | in vitro analysis, cytotoxins, assaying, methodology, environments, damage, biology, rats, chemicals, neutral, cells, enzymes, red(color), reliability, oxidation, viability, dyes, pathology, cell structure, microsomes, hepatoma cells, 1 |