[Effect of TGF-b1 siRNA-mediated silencing on Smad proteins in hepatic fibrosis rats]
Sun, YC; Liang, Q; Qian, KL; Xiao, L; Liu, Q; Shi, XF
| HERO ID | 3482489 |
|---|---|
| In Press | No |
| Year | 2012 |
| Title | [Effect of TGF-b1 siRNA-mediated silencing on Smad proteins in hepatic fibrosis rats] |
| Authors | Sun, YC; Liang, Q; Qian, KL; Xiao, L; Liu, Q; Shi, XF |
| Journal | Zhonghua Ganzangbing Zazhi |
| Volume | 20 |
| Issue | 4 |
| Page Numbers | 289-293 |
| Abstract | <strong>OBJECTIVE: </strong>To investigate the changes in Smad 2, 3, 4 and 7 of the transforming growth factor-beta 1 (TGF-b1)/Smad signaling pathways in carbon tetrachloride (CCL4)-induced hepatic fibrosis rats treated with TGF-b1 small interfering (si)RNA.<br /><br /><strong>METHODS: </strong>Rats were randomly divided among five groups: non-fibrotic (normal); fibrosis-induced (model); fibrotic treated with 0.125 mg/kg TGF-b1 siRNA; fibrotic treated with 0.250 mg/kg TGF-b1 siRNA; and fibrotic treated with negative control TGF-b1 siRNA. The expression of Smad 2, 3, 4 and 7 was detected by real-time polymerase chain reaction (for mRNA), immunohistochemistry and Western blotting (for protein).<br /><br /><strong>RESULTS: </strong>The mRNA and protein levels of Smad 2, 3 and 4 were significantly lower in the the fibrotic rats treated with either 0.250 mg/kg or 0.125 mg/kg TGF-b1 siRNA than in the fibrotic model or the negative control TGF-b1 siRNA rats (P less than 0.01). Moreover, the mRNA and protein expression levels of Smad 2, 3 and 4 were significantly lower in the 0.250 mg/kg TGF-b1 siRNA group than in the 0.125 mg/kg group (P less than 0.05). Comparing the 0.250 mg/kg and 0.125 mg/kg TGF-b1 siRNA groups to the model group and the TGF-b1 siRNA negative control group showed significantly increased levels of mRNA and protein expression of Smad 7 (P less than 0.01). In addition, the expression levels of Smad 7 were significantly higher in the 0.250 mg/kg TGF-b1 siRNA group than in the 0.125 mg/kg group (P less than 0.05).<br /><br /><strong>CONCLUSION: </strong>siRNA-mediated silencing of TGF-b1 in rats led to significantly reduced expression of Smad 2, 3 and 4, but significantly increased expression of Smad 7. TGF-b1 regulation of Smad signaling molecules may contribute to hepatic fibrosis in rats and represent a target of future therapeutic intervention. |
| Pmid | 22964151 |
| Is Certified Translation | No |
| Dupe Override | No |
| Is Public | Yes |
| Language Text | Chinese |