Evaluation of exposure biomarkers from percutaneous absorption of N-methyl-2-pyrrolidone
Akesson, B; Carnerup, MA; Jönsson, BA
| HERO ID | 3539698 |
|---|---|
| In Press | No |
| Year | 2004 |
| Title | Evaluation of exposure biomarkers from percutaneous absorption of N-methyl-2-pyrrolidone |
| Authors | Akesson, B; Carnerup, MA; Jönsson, BA |
| Journal | Scandinavian Journal of Work, Environment and Health |
| Volume | 30 |
| Issue | 4 |
| Page Numbers | 306-312 |
| Abstract | <strong>OBJECTIVES: </strong>The aim of this study was to evaluate different biomarkers of exposure to N-methyl-2-pyrrolidone (NMP), a widely used industrial chemical. For this purpose, differences in toxicokinetics between men and women and between pure and water-mixed NMP were evaluated after dermal absorption.<br /><br /><strong>METHODS: </strong>Six female and six male volunteers (groups 1 and 2) were topically exposed for 6 hours to 300 mg of NMP. An additional group of six male volunteers (group 3) was exposed to 300 mg of NMP in a 50% water solution. Blood and urine were sampled before, during, and up to 9 days after the exposure. Plasma and urine were analyzed using mass spectrometry.<br /><br /><strong>RESULTS: </strong>For groups 1 and 2, 16% and 18% of the applied dose were recovered in the urine as the sum of NMP and its metabolites. For group 3, 4% was recovered. The maximal concentration of 5-hydroxy-N-methyl-2-pyrrolidone (5-HNMP) was 10, 8.1, and 2.1 micromol/l for groups 1, 2 and 3, respectively, in plasma and 420, 360 and 62 micromol/l in urine adjusted for density. For 2-hydroxy-N-methylsuccinimide (2-HMSI), the maximal concentration was 5.4, 4.5, and 1.3 micromol/l for groups 1, 2 and 3, in plasma, respectively, and 110, 82 and 19 micromol/l in urine adjusted for density. For 5-HNMP there was a difference in time to reach the maximal concentration depending on whether pure NMP or 50% NMP in water was used. No such difference was seen for 2-HMSI. The differences in kinetics between male and female volunteers were small.<br /><br /><strong>CONCLUSIONS: </strong>Preferably 2-HMSI should be used as the biomarker of exposure to NMP. |
| Pmid | 15458014 |
| Wosid | WOS:000223735000007 |
| Is Certified Translation | No |
| Dupe Override | No |
| Is Public | Yes |
| Language Text | English |
| Keyword | biological monitoring; developmental toxicity; skin uptake; solvent |