Identification of a N7-guanine adduct of 1-bromopropane in calf thymus DNA by mass spectrometry
Thapa, P; Kim, EK; Nepal, MR; Jeong, KS; Kang, MJ; Noh, K; Lee, S; Jeong, HG; Lee, JH; Jeong, TC; Lee, ES
HERO ID
3554778
Reference Type
Journal Article
Year
2016
Language
English
| HERO ID | 3554778 |
|---|---|
| In Press | No |
| Year | 2016 |
| Title | Identification of a N7-guanine adduct of 1-bromopropane in calf thymus DNA by mass spectrometry |
| Authors | Thapa, P; Kim, EK; Nepal, MR; Jeong, KS; Kang, MJ; Noh, K; Lee, S; Jeong, HG; Lee, JH; Jeong, TC; Lee, ES |
| Journal | Molecular & Cellular Toxicology |
| Volume | 12 |
| Issue | 1 |
| Page Numbers | 7-14 |
| Abstract | As a replacement for chlorofluorocarbons that cause ozone depletion, 1-bromopropane has been widely used in work place. In the present study, the formation of N-7-guanine adduct in DNA by 1-bromopropane was evaluated in vitro to elucidate the possible mechanism of its toxic action. N-7-Propyl guanine was chemically synthesized and structurally characterized by NMR, UV, HPLC, and liquid chromatographyelectrospray ionization mass spectrometry (LC- ESI MS) for using as a reference standard. An incubation of 2'-deoxyguanosine with 1-bromopropane produced N-7-propyl adduct, which was identified by UV, HPLC and ESI-MS. In addition, N-7-guanine adduct was also identified from the incorporation of calf thymus DNA with 1-bromopropane at the physiological condition by LC-ESI MS. Furthermore, the production of adduct was proportional to the amounts of 1-bromopropane used. These results indicated that the molecular mechanism underlying toxic effects of 1-bromopropane would be associated with the adduct formation on DNA at least in part. |
| Doi | 10.1007/s13273-016-0002-5 |
| Wosid | WOS:000373095200002 |
| Is Certified Translation | No |
| Dupe Override | No |
| Is Public | Yes |
| Language Text | English |
| Keyword | 1-Bromopropane; N-7-Propyl guanine; In vitro; Calf thymus DNA; Adduct formation |