A ratiometric lysosomal pH chemosensor based on fluorescence resonance energy transfer
Fan, J; Lin, C; Li, H; Zhan, P; Wang, J; Cui, S; Hu, M; Cheng, G; Peng, X
| HERO ID | 3575457 |
|---|---|
| In Press | No |
| Year | 2013 |
| Title | A ratiometric lysosomal pH chemosensor based on fluorescence resonance energy transfer |
| Authors | Fan, J; Lin, C; Li, H; Zhan, P; Wang, J; Cui, S; Hu, M; Cheng, G; Peng, X |
| Journal | Dyes and Pigments |
| Volume | 99 |
| Issue | 3 |
| Page Numbers | 620-626 |
| Abstract | In this work, we presented a naphthalimide-rhodamine based fluorescence resonance energy transfer system (FRET) NR1 as a ratiometric and intracellular pH probe, in which 1,2,3-triazole was identified as an ideal bridge and biocompatibility. It could selectively monitor pH variations in methanol/HEPES solution at room temperature. When the pH changed from 6.20 to 2.00, both the fluorescence intensities at 580 nm and the intensity ratios, R (I-580 nm/I-538 nm) increased, which allowed the detection of pH changes by both normal fluorescence and ratiometric fluorescence methods. The observation is consistent with the increased FRET from the 1,8-naphthalimide (donor) to the ring-opened, colored form of rhodamine (acceptor). Moreover, as NR1 is lysosomal with low cytotoxicity, it will be helpful for investigating the pivotal role of H+ in a biological context, especially in lysosomes through direct intracellular imaging. (C) 2013 Elsevier Ltd. All rights reserved. |
| Doi | 10.1016/j.dyepig.2013.06.032 |
| Wosid | WOS:000327560200014 |
| Is Certified Translation | No |
| Dupe Override | No |
| Is Public | Yes |
| Keyword | Naphthalimide-rhodamine; FRET; Ratiometric sensing; pH; Fluorescent sensor; Lysosome localization |